Sánchez-Venegas Jaime R, Dinamarca Jorge, Moraga Ana Gutiérrez, Gidekel Manuel
Laboratorio de Biología Molecular Aplicada, Instituto de Agroindustrias, Facultad de Ciencias Agropecuarias y Forestales, Universidad de La Frontera, Casilla 54-D, Temuco, Chile.
BMC Res Notes. 2009 Sep 28;2:198. doi: 10.1186/1756-0500-2-198.
The Copper/Zinc superoxide dismutase (Cu/ZnSOD) gene, SOD gene, was isolated from a Deschampsia antarctica Desv. by cDNA library screening. The expression of SOD gene in the leaves of D. antarctica was determined by RT-PCR and its differential expression of gene transcripts in conditions of cold and UV radiation stresses was revealed by northern blot.
The molecular characterization shows that SOD cDNA is 709 bp in length, which translates an ORF of 152 amino acids that correspond to a protein of predicted molecular mass of 15 kDa. The assay shows that the expression of SOD gene increases when D. antarctica is acclimatised to 4 degrees C and exposed to UV radiation. These results indicate that the SOD gene of D. antarctica is involved in the antioxidative process triggered by oxidative stress induced by the conditions of environmental change in which they live.
The present results allow us to know the characteristics of Cu/ZnSOD gene from D. antarctica and understand that its expression is regulated by cold and UV radiation.
通过cDNA文库筛选，从南极发草（Deschampsia antarctica Desv.）中分离出铜/锌超氧化物歧化酶（Cu/ZnSOD）基因，即SOD基因。通过RT-PCR测定了SOD基因在南极发草叶片中的表达，并通过Northern印迹揭示了其在寒冷和紫外线辐射胁迫条件下基因转录本的差异表达。
分子特征表明，SOD cDNA长度为709 bp，其翻译出一个152个氨基酸的开放阅读框，对应于一个预测分子量为15 kDa的蛋白质。分析表明，当南极发草适应4℃并暴露于紫外线辐射时，SOD基因的表达增加。这些结果表明，南极发草的SOD基因参与了由其生存环境变化诱导的氧化应激引发的抗氧化过程。
目前的结果使我们能够了解南极发草Cu/ZnSOD基因的特征，并了解其表达受寒冷和紫外线辐射的调节。
