Sytykiewicz Hubert
Siedlce University of Natural Sciences and Humanities, Department of Biochemistry and Molecular Biology, Siedlce, Poland.
PLoS One. 2014 Apr 10;9(4):e94847. doi: 10.1371/journal.pone.0094847. eCollection 2014.
The aim of this study was to compare the expression patterns of superoxide dismutase genes (sod2, sod3.4, sod9 and sodB) in seedling leaves of the Zea mays L. Tasty Sweet (susceptible) and Ambrozja (relatively resistant) cultivars infested with one of two hemipteran species, namely monophagous Sitobion avenae F. (grain aphid) or oligophagous Rhopalosiphum padi L. (bird cherry-oat aphid). Secondarily, aphid-elicited alternations in the antioxidative capacity towards DPPH (1,1-diphenyl-2-picrylhydrazyl) radical in insect-stressed plants were evaluated. Comprehensive comparison of expression profiles of the four sod genes showed that both insect species evoked significant upregulation of three genes sod2, sod3.4 and sod9). However, aphid infestation affected non-significant fluctuations in expression of sodB gene in seedlings of both maize genotypes. The highest levels of transcript accumulation occurred at 8 h (sod2 and sod3.4) or 24 h (sod9) post-infestation, and aphid-induced changes in the expression of sod genes were more dramatic in the Ambrozja cultivar than in the Tasty Sweet variety. Furthermore, bird cherry-oat aphid colonization had a more substantial impact on levels of DPPH radical scavenging activity in infested host seedlings than grain aphid colonization. Additionally, Ambrozja plants infested by either hemipteran species showed markedly lower antioxidative capacity compared with attacked Tasty Sweet plants.
本研究的目的是比较美味甜玉米（易感品种）和安布罗齐亚玉米（相对抗性品种）幼苗叶片中超氧化物歧化酶基因（sod2、sod3.4、sod9和sodB）的表达模式，这两个品种分别受到两种半翅目昆虫之一的侵害，即单食性的麦长管蚜（禾谷缢管蚜）或寡食性的黍缢管蚜（高粱蚜）。其次，评估了蚜虫诱导的受昆虫胁迫植物对DPPH（1,1-二苯基-2-苦基肼）自由基抗氧化能力的变化。对四个sod基因表达谱的综合比较表明，两种昆虫都引起了三个基因（sod2、sod3.4和sod9）的显著上调。然而，蚜虫侵染对两种玉米基因型幼苗中sodB基因的表达产生了不显著的波动。转录积累的最高水平出现在侵染后8小时（sod2和sod3.4）或24小时（sod9），并且蚜虫诱导的sod基因表达变化在安布罗齐亚品种中比在美味甜玉米品种中更显著。此外，黍缢管蚜定殖对受侵染宿主幼苗中DPPH自由基清除活性水平的影响比禾谷缢管蚜定殖更大。此外，与受攻击的美味甜玉米植株相比，受任何一种半翅目昆虫侵染的安布罗齐亚植株的抗氧化能力明显更低。
