Mannini Antonella, Kerstin Nadja, Calorini Lido, Mugnai Gabriele, Ruggieri Salvatore
Department of Experimental Pathology and Oncology, University of Florence, Italy.
Br J Nutr. 2009 Oct;102(7):958-61. doi: 10.1017/S0007114509359139.
Epidemiological investigation and animal studies have shown that dietary n-3 PUFA prevent the development and progression of certain types of cancer. However, conflicting results have been reported by the few studies that focused on the effect of dietary n-3 PUFA on the development of metastases. In the present study, we investigated the metastatic dissemination of murine T lymphoma lines with different metastatic potential transplanted into mice fed a fish oil diet, compared with mice fed a maize oil diet. Transplantation of highly metastatic S11 cells into animals fed a fish oil diet induced a large lymphomatoid infiltration in the spleen, associated with an eight-fold increase in spleen weight, compared with normal animals on the same diet. In contrast, only a limited increase in spleen weight was found in animals transplanted with S11 cells while fed a maize oil diet. No significant increase in spleen weight was found in animals transplanted with low-metastatic 164T2 cells regardless of whether they were fed a fish oil or a maize oil diet. At the end of experiment, an overt cachexia was shown by animals fed a fish oil diet transplanted with S11 cells, but not by those transplanted with 164T2 cells. The particularly high pro-metastatic effect of dietary n-3 PUFA on S11 cells rules out the generalisation that dietary n-3 PUFA inhibit tumour growth and progression.
流行病学调查和动物研究表明，膳食中的n-3多不饱和脂肪酸（PUFA）可预防某些类型癌症的发生和发展。然而，少数关注膳食n-3 PUFA对转移瘤发生影响的研究却报告了相互矛盾的结果。在本研究中，我们将具有不同转移潜能的小鼠T淋巴瘤细胞系移植到喂食鱼油饮食的小鼠体内，并与喂食玉米油饮食的小鼠进行比较，研究其转移扩散情况。与喂食相同饮食的正常动物相比，将高转移性的S11细胞移植到喂食鱼油饮食的动物体内，会导致脾脏出现大量淋巴瘤样浸润，脾脏重量增加八倍。相比之下，在喂食玉米油饮食的情况下，移植S11细胞的动物脾脏重量仅有限增加。无论喂食鱼油还是玉米油饮食，移植低转移性164T2细胞的动物脾脏重量均未显著增加。实验结束时，喂食鱼油饮食并移植S11细胞的动物出现明显恶病质，但移植164T2细胞的动物未出现。膳食n-3 PUFA对S11细胞具有特别高的促转移作用，这排除了膳食n-3 PUFA抑制肿瘤生长和进展的普遍观点。
