Kawasoe Yasuomi, Yokouchi Masahiro, Ueno Yoshinori, Iwaya Hiroaki, Yoshida Hiroki, Komiya Setsuro
Department of Orthopaedic Surgery, Kagoshima Graduate School of Medical and Dental Sciences, Kagoshima 890-8520, Japan.
Oncol Rep. 2009 Nov;22(5):1045-50. doi: 10.3892/or_00000534.
Although hyperbaric oxygen has been shown to enhance the efficacy of radiotherapy and chemotherapy for the treatment of several malignant tumors, the impact of hyperbaric oxygen on osteosarcoma has not yet been demonstrated. In this study, we investigated the efficacy of hyperbaric oxygen alone and in combination with an anti-cancer drug as an adjuvant to chemotherapy. In vitro, highly metastatic murine osteosarcoma cell lines were exposed to hyperbaric oxygen and cell viability was examined. Hyperbaric oxygen alone significantly suppressed cell proliferation, and hyperbaric oxygen plus carboplatin exhibited significant synergism in suppression of cell proliferation. In vivo, C3H mice were subcutaneously inoculated with osteosarcoma cells and divided into four groups: control, hyperbaric oxygen, carboplatin, and carboplatin plus hyperbaric oxygen. After 5 weeks, increase in both tumor volume and number of lung metastases was significantly suppressed in the hyperbaric oxygen group. Concomitant hyperbaric oxygen clearly enhanced the chemotherapeutic effects of carboplatin on both tumor growth and lung metastasis in osteosarcoma-bearing mice. Moreover, mortality in the carboplatin plus hyperbaric oxygen group was significantly lower than in the other three groups. These findings suggest that hyperbaric oxygen plus carboplatin combination therapy could be an appropriate therapeutic regimen for the treatment of patients with osteosarcoma.
尽管高压氧已被证明可提高放疗和化疗对几种恶性肿瘤的治疗效果，但高压氧对骨肉瘤的影响尚未得到证实。在本研究中，我们调查了单独使用高压氧以及将其与抗癌药物联合作为化疗辅助手段的疗效。在体外，将高转移性小鼠骨肉瘤细胞系暴露于高压氧环境中，并检测细胞活力。单独使用高压氧可显著抑制细胞增殖，高压氧联合卡铂在抑制细胞增殖方面表现出显著的协同作用。在体内，将骨肉瘤细胞皮下接种于C3H小鼠，并分为四组：对照组、高压氧组、卡铂组和卡铂加高压氧组。5周后，高压氧组的肿瘤体积和肺转移数量的增加均受到显著抑制。同时，高压氧明显增强了卡铂对荷骨肉瘤小鼠肿瘤生长和肺转移的化疗效果。此外，卡铂加高压氧组的死亡率显著低于其他三组。这些发现表明，高压氧联合卡铂的联合治疗可能是治疗骨肉瘤患者的合适治疗方案。
