Department of Animal Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620024, India.
J Physiol Sci. 2010 Jan;60(1):43-9. doi: 10.1007/s12576-009-0064-4. Epub 2009 Sep 29.
The COP9 signalosome (CSN) is a multi-subunit protein complex conserved in plants and animals. CSN subunits have been identified as light-mediated master regulators of eukaryotic circadian clocks from fungi to animals. The Indian false vampire bat Megaderma lyra is completely adapted to an anthropic biotope and behavioral studies have reported that M. lyra exhibits light-sampling behavior to assess environmental light. LC-MS-MS results for a 36 kDa protein were analyzed using the Sequest search engine, and COP9 signalosome subunit 5 (CSN5) was pinpointed as having the highest score with 6 matching peptides. To confirm the presence of CSN5, up-regulated cDNA was amplified, sequenced, and identified as CSN5. Furthermore, semi-quantitative RT-PCR analysis demonstrated that the level of induction of CSN5 was regulated by environmental light. We estimated the level of expression across a light-dark cycle and observed a higher level of expression at the end of the light phase. Similarly, when the animal was shifted from continuous dark to light, CSN5 expression was induced. Correspondingly, we detected the similar pattern of translated protein with JAB1 antibody. Knowledge about the circadian rhythm and its molecular mechanism in Chiroptera is very limited and this study suggests that CSN5 might be involved in the M. lyra light-signaling process.
COP9 信号体（CSN）是一种在植物和动物中保守的多亚基蛋白复合物。CSN 亚基已被鉴定为从真菌到动物的真核生物钟光介导的主要调节因子。印度假吸血蝙蝠 Megaderma lyra 完全适应了人为生物群落，行为研究报告称 M. lyra 表现出光采样行为以评估环境光。使用 Sequest 搜索引擎对 36 kDa 蛋白的 LC-MS-MS 结果进行了分析，COP9 信号体亚基 5（CSN5）被确定为得分最高的蛋白，有 6 个匹配肽。为了确认 CSN5 的存在，扩增了上调的 cDNA，测序并鉴定为 CSN5。此外，半定量 RT-PCR 分析表明，CSN5 的诱导水平受环境光调节。我们在光-暗周期内估计了表达水平，并在光相结束时观察到更高的表达水平。同样，当动物从连续黑暗转为光照时，CSN5 的表达被诱导。相应地，我们用 JAB1 抗体检测到了类似的翻译蛋白模式。关于蝙蝠的昼夜节律及其分子机制的知识非常有限，本研究表明 CSN5 可能参与了 M. lyra 的光信号转导过程。
