Stelzer Rosemary, Hutz Reinhold J
Department of Biological Sciences, University of Wisconsin-Milwaukee, WI 53211, USA.
J Reprod Dev. 2009 Dec;55(6):685-90. doi: 10.1262/jrd.20241. Epub 2009 Sep 29.
Nanoparticle technology refers to research and technology developed at the atomic or molecular level for materials of approximately 1-100 nm in length. Through accidental or involuntary exposure, nanoparticles are potentially toxic to the body, including reproductive organs. Ovarian granulosa cells play a major role in maintaining ovarian function, health, and female fertility. Since these cells are involved in steroidogenesis, we wished to evaluate whether nanoparticles affected them after traversing their membranes. Cells were co-incubated with 10 nm gold particles for up to 24 h. Transmission electron micrographs were taken of GC treated with 10 nm gold particles in order to compare and contrast ultrastructural locations of nanoparticles with treatment. From micrograph comparisons of treated vs. untreated GC at various culture times, it appeared that some intracellular organelles involved in steroidogenesis were infiltrated and/or altered due to the presence of the nanogold particles. Medium samples were taken in order to determine estradiol-17beta (E2) accumulation/secretion by untreated vs. treated cells. GC incubated with 10 nm nanogold particles for 1, 3, or 5 h were found to accumulate significantly increased amounts of estrogen compared with untreated cells. Conversely, at 24 h there was a significant attenuation with respect to controls. The data presented here provide insight into the toxicologic effects gold nanoparticles elicit on ovarian granulosa cells.
纳米颗粒技术是指在原子或分子水平上针对长度约为1-100纳米的材料所开展的研究与技术。通过意外或非自愿接触，纳米颗粒可能对身体有毒，包括对生殖器官。卵巢颗粒细胞在维持卵巢功能、健康和女性生育能力方面发挥着主要作用。由于这些细胞参与类固醇生成，我们希望评估纳米颗粒穿过其细胞膜后是否会对它们产生影响。细胞与10纳米的金颗粒共同孵育长达24小时。对用10纳米金颗粒处理的颗粒细胞进行透射电子显微镜拍照，以便比较和对照纳米颗粒在处理后的超微结构位置。从不同培养时间处理过的颗粒细胞与未处理的颗粒细胞的显微照片比较来看，似乎一些参与类固醇生成的细胞内细胞器因纳米金颗粒的存在而受到浸润和/或改变。采集培养基样本以确定未处理细胞与处理过的细胞中雌二醇-17β（E2）的积累/分泌情况。与未处理的细胞相比，发现与10纳米纳米金颗粒孵育1、3或5小时的颗粒细胞积累的雌激素量显著增加。相反，在24小时时，与对照组相比有显著衰减。此处呈现的数据为金纳米颗粒对卵巢颗粒细胞产生的毒理学效应提供了见解。
