Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Spl. Independentei, 050095, Bucharest, Romania.
Arch Environ Contam Toxicol. 2010 Apr;58(3):757-64. doi: 10.1007/s00244-009-9401-0. Epub 2009 Sep 30.
Pyrethroids, such as deltamethrin, are toxic substances that lead to generation of reactive oxygen species, which harm living organisms. We assessed the level and patterns of imbalance evolved by a single dose of 2 microg/L deltamethrin on the lipid peroxidation (LPO) and the antioxidant defense system of Carassius auratus gibelio liver and intestine, and monitored the recovery dynamics of these parameters during a 14-day post-exposure period. LPO and antioxidative defense mechanisms displayed different responses in the investigated tissues. Sudden increase of LPO in the liver, persisting at this elevated level throughout the test period, was observed on the third day post-exposure, while in the intestine significant enhancement of this parameter was recorded from the seventh day. Reduced glutathione (GSH) showed a transient increase in the liver, and was depleted in the intestine by the second day of exposure, with signs of recovery by the end of the experimental tenure. In the liver of fish a temporary inhibition of superoxide dismutase (SOD) and catalase (CAT) activity, and activation of glutathione peroxidase (GPX), glutathione transferase (GST), and glutathione reductase (GR) enzymes was observed, with maximum thresholds recorded on the third and second days, respectively. In the intestine a relevant increase in CAT and GST activity up to the second day and almost complete recovery by the end of the experiment was recorded, while for GR a continuous enhancement was apparent.
拟除虫菊酯，如溴氰菊酯，是有毒物质，会导致活性氧的产生，从而损害生物体。我们评估了 2 微克/升溴氰菊酯单剂量对鲫鱼肝脏和肠道脂质过氧化（LPO）和抗氧化防御系统的失衡水平和模式，并监测了这些参数在 14 天暴露后期间的恢复动态。在研究的组织中，LPO 和抗氧化防御机制表现出不同的反应。暴露后第三天，肝脏中 LPO 突然增加，并在整个测试期间持续维持在这个升高的水平，而在肠道中，从第七天开始记录到这个参数的显著增强。在肝脏中，还原型谷胱甘肽（GSH）短暂增加，在暴露的第二天在肠道中耗尽，在实验结束时出现恢复迹象。在鱼的肝脏中，观察到超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性的暂时抑制，以及谷胱甘肽过氧化物酶（GPX）、谷胱甘肽转移酶（GST）和谷胱甘肽还原酶（GR）酶的激活，最大阈值分别记录在第三天和第二天。在肠道中，CAT 和 GST 活性在第二天增加到最高，在实验结束时几乎完全恢复，而 GR 则持续增强。
