Equipe Environnement et Microbiologie-IPREM UMR 5254-IBEAS, Université de Pau et des Pays de l'Adour, Avenue de l'Université, BP 1155, 64013 Pau Cedex, France.
Microb Ecol. 2010 Apr;59(3):445-56. doi: 10.1007/s00248-009-9585-x. Epub 2009 Sep 30.
In order to estimate how pollution affects the bacterial community structure and composition of sediments, chemical and molecular approaches were combined to investigate eight stations around the Bizerte lagoon. Terminal restriction fragment length polymorphism (T-RFLP) analysis of PCR-amplified 16S rRNA genes revealed that each station was characterized by a specific bacterial community structure. The combination of this data with those of chemical analysis showed a correlation between the bacterial fingerprint and the pollutant content, principally with hydrocarbon pollution. The composition of the bacterial community of two contrasted stations related to the pollution revealed sequences affiliated to alpha, beta, gamma, delta, epsilon subclass of the Proteobacteria, Actinobacteria, and Acidobacteria in both stations although in different extent. Gamma and delta subclass of the Proteobacteria were dominant and represent 70% of clones in the heavy-metal-contaminated station and 47% in the polyaromatic hydrocarbon (PAH)-contaminated. Nevertheless, most of the sequences found were unaffiliated to cultured bacteria. The adaptation of the bacterial community mainly to PAH compounds demonstrated here and the fact that these bacterial communities are mainly unknown suggest that the Bizerte lagoon is an interesting environment to understand the capacity of bacteria to cope with some pollutants.
为了评估污染对沉积物中细菌群落结构和组成的影响，我们结合化学和分子方法，调查了比塞大泻湖周围的 8 个站点。聚合酶链反应（PCR）扩增的 16S rRNA 基因的末端限制性片段长度多态性（T-RFLP）分析表明，每个站点的细菌群落结构都具有特异性。将这些数据与化学分析数据相结合，表明细菌指纹图谱与污染物含量之间存在相关性，主要与碳氢化合物污染有关。对两个受污染程度不同的对比站点的细菌群落组成的研究揭示了与 alpha、beta、gamma、delta、epsilon 亚群的 Proteobacteria、Actinobacteria 和 Acidobacteria 有关的序列，尽管在不同程度上存在差异。在重金属污染的站点中，gamma 和 delta 亚群的 Proteobacteria 占主导地位，占克隆的 70%，在多环芳烃（PAH）污染的站点中占 47%。然而，大多数发现的序列与培养细菌无关。这里主要展示了细菌群落对 PAH 化合物的适应能力，以及这些细菌群落主要是未知的事实，这表明比塞大泻湖是一个很有趣的环境，可以了解细菌应对某些污染物的能力。
