Todorova Nadezhda H, Mironova Roumyana S, Karamfilov Ventzislav K
Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Y. Gagarin 2 str., 1113 Sofia, Bulgaria.
Institute of Molecular Biology "Roumen Tsanev", Bulgarian Academy of Sciences, G. Bonchev str., bl. 21, 1113 Sofia, Bulgaria.
Mar Pollut Bull. 2014 Jun 15;83(1):231-40. doi: 10.1016/j.marpolbul.2014.03.047. Epub 2014 Apr 20.
Molecular analysis was applied to characterize bacterial community structure in sediment samples collected from pristine site and oil-polluted Black Sea harbor. Amplified Ribosomal DNA Restriction Analysis (ARDRA) revealed a high similarity in the restriction patterns of both samples thus not demonstrating the effect of the pollutant on the structure of the bacterial communities. Constructed 16S rRNA gene libraries gave more detailed assessment of members. Results showed that α- and γ-Proteobacteria were dominant in the oil polluted site, whereas the pristine site was characterized by prevalence of Actinobacteria. The biodegradative potential of the adapted bacterial community in the oil-polluted sediments was demonstrated by the presence of the aromatic ring hydroxylating dioxygenase genes.
应用分子分析方法对从原始地点和受石油污染的黑海港口采集的沉积物样本中的细菌群落结构进行表征。扩增核糖体DNA限制性分析（ARDRA）显示，两个样本的限制性图谱具有高度相似性，因此未证明污染物对细菌群落结构的影响。构建的16S rRNA基因文库对群落成员进行了更详细的评估。结果表明，α-和γ-变形菌纲在受石油污染的地点占主导地位，而原始地点的特征是放线菌占优势。石油污染沉积物中适应的细菌群落的生物降解潜力通过芳香环羟基化双加氧酶基因的存在得以证明。
