Human Gene Therapy Division, Faculty of Medicine, Akdeniz University, Antalya, 07070, Turkey.
Mol Imaging Biol. 2010 Jun;12(3):278-85. doi: 10.1007/s11307-009-0260-x. Epub 2009 Sep 30.
We tested a new light detection cooled charge-coupled device (CCD) for in vivo assessment of noninvasive, whole-body fluorescence optical imaging of adenovirus directed enhanced green fluorescent protein (AdEGFP) expression.
AdEGFP was injected i.v. into BALB/c mice via tail vein. Whole-body fluorescence optical imaging of AdEGFP expression was performed using a Kodak 2000MM Image Station before and after vector administration.
EGFP expression was exclusively detected around the abdominal cavity, and the fluorescent signal peaked at day 4 and then remained detectable for at least 30 days. Ex vivo fluorescence imaging confirmed that EGFP expression was restricted to the liver, and transgene expression was homogeneously diffused into all four lobes.
These findings demonstrate that in vivo fluorescence imaging provides functional data indicating the approximate location, magnitude, and duration of AdEGFP expression.
我们测试了一种新的光探测冷却电荷耦合器件（CCD），用于非侵入性、全身荧光光学成像，以评估腺病毒介导的增强型绿色荧光蛋白（AdEGFP）的表达。
通过尾静脉向 BALB/c 小鼠静脉内注射 AdEGFP。在给予载体前后，使用 Kodak 2000MM 图像工作站进行 AdEGFP 表达的全身荧光光学成像。
EGFP 表达仅在腹腔周围被检测到，荧光信号在第 4 天达到峰值，然后至少在 30 天内仍可检测到。离体荧光成像证实 EGFP 表达仅限于肝脏，并且转基因表达均匀地扩散到四个叶。
这些发现表明，体内荧光成像提供了功能数据，表明 AdEGFP 表达的大致位置、程度和持续时间。
