Planta Med. 2010 Mar;76(4):399-401. doi: 10.1055/s-0029-1186161. Epub 2009 Sep 29.
Molecular identification of PANAX GINSENG (Korean ginseng) cultivars is very important for their management. We have developed a simple and reliable method for specific identification of the superior cultivar "Gumpoong." The 26S rDNA was targeted for our molecular analysis, and a single-nucleotide polymorphism (SNP) was identified between Gumpoong and the other cultivars within the sequence data. From this SNP site, a modified allele-specific primer and a novel primer set have been developed to identify the Gumpoong cultivar via multiplex PCR. The established multiplex PCR method was determined to be effective, and the SNP marker showed high specificity to Gumpoong. Therefore, the described method may serve as a useful tool for rapid selection of Gumpoong cultivar.
人参（韩国高丽参）品种的分子鉴定对于其管理非常重要。我们已经开发出一种简单可靠的方法，用于特异性鉴定优良品种“金刚”。我们的分子分析以 26S rDNA 为目标，并在序列数据中鉴定出金刚与其他品种之间的单核苷酸多态性（SNP）。从这个 SNP 位点，开发了一种改良的等位基因特异性引物和一种新的引物组，通过多重 PCR 来鉴定金刚品种。所建立的多重 PCR 方法是有效的，SNP 标记对金刚具有高度特异性。因此，所描述的方法可以作为快速选择金刚品种的有用工具。
