利用线粒体nad7内含子4区域对高丽参品种“春坪”进行分子鉴定。
Molecular identification of the Korean ginseng cultivar "Chunpoong" using the mitochondrial nad7 intron 4 region.
作者信息
Wang Hongtao, Sun Hua, Kwon Woo-Saeng, Jin Haizhu, Yang Deok-Chun
机构信息
Korean Ginseng Center for Most Valuable Products and Ginseng Genetic Resource Bank, Kyung Hee University, Yongin, Gyunggi-do, Republic of Korea.
出版信息
Mitochondrial DNA. 2009 Apr-Jun;20(2-3):41-5. doi: 10.1080/19401730902856738.
BACKGROUND AND AIMS
Korean ginseng (Panax ginseng) is one of the most important medicinal plants in the Orient. Among the nine cultivars of Korean ginseng, "Chunpoong" commands a much greater market value and has been planted widely.
MATERIALS AND METHODS
A rapid and reliable method for discriminating the Chunpoong cultivar was developed by exploiting a single nucleotide polymorphism (SNP) in the mitochondrial nad7 intron 4 region of nine Korean ginseng cultivars using universal primers.
RESULTS
A SNP was detected between Chunpoong and other cultivars, and modified allele-specific primers were designed from this SNP site to specifically identify Chunpoong cultivar via multiplex PCR.
CONCLUSION
We therefore present an effective method for the genetic identification of the Chunpoong cultivar of ginseng.
背景与目的
高丽参（人参）是东方最重要的药用植物之一。在高丽参的九个栽培品种中，“春棚”具有更高的市场价值且种植广泛。
材料与方法
利用通用引物，通过挖掘九个高丽参栽培品种线粒体nad7内含子4区域的单核苷酸多态性（SNP），开发了一种快速可靠的鉴别春棚品种的方法。
结果
在春棚与其他品种之间检测到一个SNP，并从该SNP位点设计了修饰的等位基因特异性引物，通过多重PCR特异性鉴定春棚品种。
结论
因此，我们提出了一种有效的人参春棚品种基因鉴定方法。
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