Liu Y G, Mori N, Tsunewaki K
Laboratory of Genetics, Faculty of Agriculture, Kyoto University, Japan.
Jpn J Genet. 1990 Oct;65(5):367-80. doi: 10.1266/jjg.65.367.
To develop detailed linkage maps of restriction fragment length polymorphism (RFLP) sites in wheat chromosomes, it was necessary to construct a genomic DNA library and to characterize the clones obtained. Forty-nine per cent of the clones were of single or low copy number per genome. With 91 clones of this class, as probes, and with two to four restriction endonucleases, for DNA digestion, RFLPs were examined among eight common wheats and a single emmer wheat. About 20% of the probes, and 13% of the probe-enzyme combinations revealed genetic polymorphism among the common wheats. DNA deletions account for most of the genetic differences among these wheat genomes. Based on the RFLP data, phylogenetic distances among the nine polyploid wheats were estimated, and a dendrogram showing the genetic relationships among them was constructed.
为了绘制小麦染色体上限制片段长度多态性（RFLP）位点的详细连锁图谱，有必要构建一个基因组DNA文库并对获得的克隆进行表征。49%的克隆在每个基因组中为单拷贝或低拷贝数。以这类的91个克隆作为探针，并使用两到四种限制性内切酶进行DNA消化，在8个普通小麦和1个二粒小麦中检测RFLP。约20%的探针以及13%的探针-酶组合在普通小麦中显示出遗传多态性。DNA缺失是这些小麦基因组间大部分遗传差异的原因。基于RFLP数据，估计了9个多倍体小麦之间的系统发育距离，并构建了显示它们之间遗传关系的树状图。
