Davis S D, Sarff L D, Hyndiuk R A
Arch Ophthalmol. 1977 Sep;95(9):1638-43. doi: 10.1001/archopht.1977.04450090160016.
Antibiotic therapy of experimental Pseudomonas keratitis was evaluated quantitatively by determining numbers of viable bacteria in the cornea of guinea pigs. Topically applied carbenicillin disodium, gentamicin sulfate, and tobramycin sulfate were often significantly more effective than topically applied polymyxin B sulfate. Intramuscular therapy with tobramycin was as effective as topical therapy, and the results exhibited less variability. Topical tobramycin every 30 minutes was significantly more effective than topical therapy every 60 minutes. No combination of antibiotics was significantly better than a single effective drug. The concentration of tobramycin in the aqueous correlated more closely to therapeutic efficacy than did the concentration in the cornea. Although all antibiotics reduced numbers of bacteria in the cornea by more than 99% in the first 24 hours of therapy, none was able to sterilize the cornea in four additional days of continuous therapy. Persistence of organisms despite apparently adequate topical therapy may explain some reported cases of relapse in humans.
通过测定豚鼠角膜中活细菌的数量，对实验性铜绿假单胞菌角膜炎的抗生素治疗进行了定量评估。局部应用的羧苄青霉素二钠、硫酸庆大霉素和硫酸妥布霉素通常比局部应用的硫酸多粘菌素B显著更有效。肌肉注射妥布霉素与局部治疗效果相同，且结果的变异性较小。每30分钟局部应用妥布霉素比每60分钟局部治疗显著更有效。没有一种抗生素组合比单一有效药物显著更好。房水中妥布霉素的浓度与治疗效果的相关性比角膜中的浓度更密切。尽管在治疗的头24小时内所有抗生素都使角膜中的细菌数量减少了99%以上，但在连续治疗的另外四天中，没有一种抗生素能够使角膜灭菌。尽管局部治疗明显充分，但细菌仍持续存在，这可能解释了一些人类复发病例的报道。
