Simmonds P, Zhang L Q, Watson H G, Rebus S, Ferguson E D, Balfe P, Leadbetter G H, Yap P L, Peutherer J F, Ludlam C A
Department of Medical Microbiology, University of Edinburgh, UK.
Lancet. 1990 Dec 15;336(8729):1469-72. doi: 10.1016/0140-6736(90)93179-s.
The polymerase chain reaction (PCR) detected specific hepatitis C viral (HCV) RNA sequences in plasma from 15 of 21 haemophiliacs (12 HCV-antibody positive) and 7 of 27 intravenous drug users (13 HCV-antibody positive). Quantification of RNA-positive samples showed high levels of HCV (10(5) to 10(6) copies of RNA/ml) in infected patients. HCV was more frequently found in haemophiliacs infected with human immunodeficiency virus (11/11 HIV-positive and 4/10 HIV-negative patients). HCV-RNA was detected in all batches of commercially available factor VIII tested and in low concentrations in some pools of plasma donations from volunteers. Factor VIII, manufactured from volunteer donations, was uniformly negative by PCR. Phylogenetic analysis of viral sequences showed two distinct groups: one was associated with intravenous drug users and the other with haemophiliacs infected with Scottish factor VIII preparations. Both were distinct from sequences found in commercially available factor VIII.
聚合酶链反应（PCR）在21名血友病患者中的15名（12名丙肝抗体阳性）以及27名静脉吸毒者中的7名（13名丙肝抗体阳性）的血浆中检测到了特定的丙型肝炎病毒（HCV）RNA序列。对RNA阳性样本的定量分析显示，感染患者体内的HCV水平很高（每毫升10⁵至10⁶个RNA拷贝）。在感染人类免疫缺陷病毒的血友病患者中更频繁地发现HCV（11名HIV阳性患者中的11名以及10名HIV阴性患者中的4名）。在所检测的所有批次市售凝血因子VIII以及来自志愿者的一些血浆捐献池中的低浓度样本中均检测到了HCV-RNA。由志愿者捐献制备的凝血因子VIII经PCR检测均呈阴性。病毒序列的系统发育分析显示出两个不同的组：一组与静脉吸毒者相关，另一组与感染了苏格兰凝血因子VIII制剂的血友病患者相关。两者均与市售凝血因子VIII中发现的序列不同。
