Lu Yin, Chen Shaoyuan, Wang Ben
College of Biological and Environmental Engineering, Zhejiang Shuren University, Hangzhou, P. R. China.
Z Naturforsch C J Biosci. 2009 Jul-Aug;64(7-8):518-20. doi: 10.1515/znc-2009-7-809.
Eleven strains of endophytic fungi which habitat in an endangered, Chinese endemic medicinal plant, Dysosma pleiantha (Hance) Woodson, were isolated and tested for their cytotoxic activity using the brine shrimp lethality bioassay. Six isolates were found to exhibit some cytotoxic activity. Extracts of F1273, F1276, and F1280, which were identified as Trichoderma citrinoviride, Chaetomium globosum and Ascomycete sp., in particular, showed most potent activity with LC50 values of 4.86, 7.71, and 14.88 microg/ml, respectively. These results indicate that endophytic fungi of Dysosma pleiantha could be a promising source for antitumour agents.
从中国特有的濒危药用植物六角莲（Dysosma pleiantha (Hance) Woodson）中分离出11株内生真菌，并采用卤虫致死生物测定法检测其细胞毒性活性。发现6株分离物具有一定的细胞毒性活性。特别是被鉴定为黄绿木霉（Trichoderma citrinoviride）、球毛壳菌（Chaetomium globosum）和子囊菌（Ascomycete sp.）的F1273、F1276和F1280提取物表现出最强的活性，LC50值分别为4.86、7.71和14.88微克/毫升。这些结果表明，六角莲内生真菌可能是抗肿瘤药物的一个有前景的来源。
