Rheinstädter Maikel C, Schmalzl Karin, Wood Kathleen, Strauch Dieter
Department of Physics and Astronomy, McMaster University, Hamilton, Ontario, L8S 4M1, Canada.
Phys Rev Lett. 2009 Sep 18;103(12):128104. doi: 10.1103/PhysRevLett.103.128104.
We present experimental evidence for a long-range protein-protein interaction in purple membrane (PM). The interprotein dynamics were quantified by measuring the spectrum of the acoustic phonons in the 2D bacteriorhodopsin (BR) protein lattice using inelastic neutron scattering. Phonon energies of about 1 meV were determined. The data are compared to an analytical model, and the effective spring constant for the interaction between neighboring protein trimers are determined to be k = 53 N/m. Additional, optical-like excitations at 0.45 meV were found and assigned to intraprotein dynamics between neighboring BR monomers.
我们提供了紫色膜（PM）中远距离蛋白质-蛋白质相互作用的实验证据。通过使用非弹性中子散射测量二维细菌视紫红质（BR）蛋白质晶格中的声子光谱，对蛋白质间动力学进行了量化。确定了约1毫电子伏特的声子能量。将数据与分析模型进行比较，确定相邻蛋白质三聚体之间相互作用的有效弹簧常数为k = 53 N/m。此外，还发现了0.45毫电子伏特处类似光学的激发，并将其归因于相邻BR单体之间的蛋白质内动力学。
