Niessen Katherine A, Xu Mengyang, Markelz A G
Department of Physics, University at Buffalo (SUNY), Buffalo, NY, 14260, USA.
Biophys Rev. 2015 Jun;7(2):201-216. doi: 10.1007/s12551-015-0168-4. Epub 2015 Apr 7.
A suggested mechanism for allosteric response is the distortion of the energy landscape with agonist binding changing the protein structure's access to functional configurations. Intramolecular vibrations are indicative of the energy landscape and may have trajectories that enable functional conformational change. Here, we discuss the development of an optical method to measure the intramolecular vibrations in proteins, namely, crystal anisotropy terahertz microscopy, and the various approaches which can be used to identify the spectral data with specific structural motions.
一种变构反应的推测机制是，激动剂结合改变蛋白质结构进入功能构型的途径，从而使能量景观发生扭曲。分子内振动指示能量景观，并且可能具有能够实现功能构象变化的轨迹。在此，我们讨论一种用于测量蛋白质分子内振动的光学方法的发展，即晶体各向异性太赫兹显微镜，以及可用于将光谱数据与特定结构运动相关联的各种方法。
