Kameda T, Yasui W, Yoshida K, Tsujino T, Nakayama H, Ito M, Ito H, Tahara E
First Department of Pathology, Hiroshima University School of Medicine, Japan.
Cancer Res. 1990 Dec 15;50(24):8002-9.
The expression of p185ERBB2 in a total of 34 human gastric carcinoma tissues as well as in corresponding normal mucosa was examined by Western blotting. More than 70% of both tumor tissues and normal mucosa showed p185ERBB2 expression at various levels. Eighteen (55%) cases revealed higher levels of p185ERBB2 in the tumor than in normal mucosa, while 13 (38%) cases showed lower levels in the tumor tissues. Higher expression of p185ERBB2 was frequently observed in well differentiated adenocarcinomas, with the incidence between well differentiated type and poorly differentiated type being significantly different (P less than 0.05). Comparative immunohistochemical analysis revealed the consistent results with p185ERBB2 expression obtained by Western blotting in well differentiated adenocarcinomas. Of the 34 cases, three well differentiated adenocarcinomas had extremely high levels of p185ERBB2. ERBB2 gene was amplified in two of the three tumors, but the amplification differed by the tumor site from where the sample was obtained. Another tumor which showed an extremely high level of p185ERBB2 but no gene amplification demonstrated a high level of binding protein to the TATA box that is located in the promoter of the ERBB2 gene. A high level of TATA-binding protein was also detected in gastric carcinoma cell lines which contain a single copy of ERBB2 gene and a high expression of p185ERBB2.
采用蛋白质免疫印迹法检测了34例人胃癌组织及其相应正常黏膜组织中p185ERBB2的表达情况。超过70%的肿瘤组织和正常黏膜组织均呈现出不同水平的p185ERBB2表达。18例（55%）病例显示肿瘤组织中p185ERBB2水平高于正常黏膜，而13例（38%）病例肿瘤组织中该蛋白水平较低。高分化腺癌中经常观察到p185ERBB2的高表达，高分化型与低分化型之间的发生率有显著差异（P<0.05）。免疫组织化学对比分析显示，高分化腺癌中p185ERBB2的表达结果与蛋白质免疫印迹法一致。34例病例中，3例高分化腺癌的p185ERBB2水平极高。这3例肿瘤中有2例ERBB2基因发生扩增，但扩增情况因样本获取的肿瘤部位而异。另一例p185ERBB2水平极高但无基因扩增的肿瘤，其与位于ERBB2基因启动子区域的TATA盒结合蛋白水平较高。在含有单拷贝ERBB2基因且p185ERBB2高表达的胃癌细胞系中也检测到了高水平的TATA结合蛋白。
