McGill K, Kelly L, Madden R H, Moran L, Carroll C, O'Leary A, Moore J E, McNamara E, O'Mahony M, Fanning S, Whyte P
Centre for Food Safety, School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
J Microbiol Methods. 2009 Nov;79(2):238-41. doi: 10.1016/j.mimet.2009.09.020. Epub 2009 Sep 28.
The antibiotic resistance profiles of 75 Campylobacter isolates of food and human clinical origin was determined by two agar diffusion susceptibility methods; disc diffusion and epsilometer-test (E-test). The most common therapeutic antimicrobials, erythromycin, ciprofloxacin and tetracycline were studied, along with chloramphenicol, ampicillin and naladixic acid. The resistance observed for each antimicrobial, as determined by both of methods, were statistically compared using Fisher two-tailed analysis. Of the six antimicrobials studied only two were shown to have statistically different patterns when resistance was compared by disc diffusion and E-test. The percentage of isolates resistant to clinically relevant antimicrobials using both techniques ranged from 6.6 to 21.3% for erythromycin, 25.3-26.6% for tetracycline and 33.3-36.0% for ciprofloxacin. The prevalence of multi-drug resistant (MDR) campylobacters (isolates resistant to 2 or more antimicrobials) for both disc diffusion and E-test was 44%. It can be concluded that, for four of the six antimicrobials assessed, antimicrobial resistance prevalences could be equally determined by either of the methods studied.
采用纸片扩散法和梯度稀释法（E-test）这两种琼脂扩散药敏试验方法，对75株来源于食品和人类临床的弯曲杆菌分离株的抗生素耐药谱进行了测定。研究了最常用的治疗性抗菌药物红霉素、环丙沙星和四环素，以及氯霉素、氨苄西林和萘啶酸。使用Fisher双尾分析对两种方法测定的每种抗菌药物的耐药性进行统计学比较。在研究的六种抗菌药物中，只有两种在通过纸片扩散法和E-test法比较耐药性时显示出统计学上不同的模式。使用两种技术对临床相关抗菌药物耐药的分离株百分比，红霉素为6.6%至21.3%，四环素为25.3%至26.6%，环丙沙星为33.3%至36.0%。纸片扩散法和E-test法检测的多重耐药（MDR）弯曲杆菌（对两种或更多种抗菌药物耐药的分离株）的患病率均为44%。可以得出结论，对于所评估的六种抗菌药物中的四种，两种研究方法均可同等确定抗菌药物耐药率。
