Tsuzaki K, Kotani K, Nagai N, Saiga K, Sano Y, Hamada T, Moritani T, Yoshimura M, Egawa K, Horikawa C, Kitagawa Y, Kiso Y, Sakane N
Division of Preventive Medicine and Diabetes Education, Clinical Research Institute for Endocrine and Metabolic Disease, National Hospital Organization Kyoto Medical Center, Kyoto, Japan.
J Endocrinol Invest. 2009 May;32(5):395-400. doi: 10.1007/BF03346474.
In the adiponectin gene polymorphisms, single-nucleotide polymorphism (SNP)-45 and SNP276 have reportedly been associated with obesity, Type 2 diabetes, and other features of metabolic syndrome.
Whether these adiponectin SNP affect obesity-related parameters during caloric restriction in obese subjects.
Thirty- two obese Japanese women were treated by meal replacement with a low calorie diet for 8 weeks and asked to maintain their habitual lifestyle. Obesity-related parameters were measured before and after the treatment period. We determined four SNP (T45G, I164T, G276T, and C-11377G) using a fluorescent allele-specific DNA primer assay systemand FRET probe assay system.
After the treatment, the extent of decrease in waist circumference was greater in the subjects with the G/G or G/T genotype of SNP276 than in those with the T/T genotype (p=0.026). As for SNP45, the extent of decrease in triglyceride levels was greater in the subjects with the T/T genotype than in those with the T/G genotype (p=0.003). For SNP-11377, the extent of decrease in systolic blood pressure and fasting plasma glucose was greater in the subjects with the C/G or G/G genotype than in those with the C/C genotype (p=0.044).
Our findings indicate that each SNP in the adiponectin gene might modify the change in obesity-related parameters during meal replacement with a low calorie diet.
在脂联素基因多态性中，据报道单核苷酸多态性（SNP）-45和SNP276与肥胖、2型糖尿病及代谢综合征的其他特征有关。
探讨这些脂联素SNP在肥胖受试者热量限制期间是否会影响与肥胖相关的参数。
32名肥胖日本女性采用低热量饮食代餐治疗8周，并要求维持其习惯性生活方式。在治疗前后测量与肥胖相关的参数。我们使用荧光等位基因特异性DNA引物分析系统和荧光共振能量转移（FRET）探针分析系统测定了4个SNP（T45G、I164T、G276T和C-11377G）。
治疗后，SNP276的G/G或G/T基因型受试者的腰围减小程度大于T/T基因型受试者（p = 0.026）。对于SNP45，T/T基因型受试者的甘油三酯水平下降程度大于T/G基因型受试者（p = 0.003）。对于SNP-11377，C/G或G/G基因型受试者的收缩压和空腹血糖下降程度大于C/C基因型受试者（p = 0.044）。
我们的数据表明，脂联素基因中的每个SNP可能会改变低热量饮食代餐期间与肥胖相关参数的变化。
