在突尼斯志愿者中，脂联素 8 种多态性与肥胖和代谢综合征参数之间的关联。
Association between eight adiponectin polymorphisms, obesity, and metabolic syndrome parameters in Tunisian volunteers.
机构信息
Biochemistry Department, Sahloul University Hospital, Sousse, Tunisia.
出版信息
Metab Syndr Relat Disord. 2011 Dec;9(6):419-26. doi: 10.1089/met.2011.0035. Epub 2011 Aug 26.
BACKGROUND
Adiponectin is a plasma protein produced by the adipose tissue, with insulin sensibility, antiinflammatory and antiatherogenic properties. Many adiponectin gene polymorphisms have been described, and their implication in obesity, metabolic syndrome, and cardiovascular diseases was controversial. Our aim was to study the relationship between eight adiponectin polymorphisms (-1391G/A, -1377C/G, 4522C/T, 395 G/A, 276G/T, 639C/T, 45T/G, and +2019delA), metabolic syndrome parameters, and the risk of obesity in Tunisian volunteers.
METHODS
We have recruited 169 nonobese [sex ratio=0.594, mean age 43.25±13.12 years; mean body mass index (BMI) 24.73±3.50 kg/m(2)] and 160 obese (BMI≥30 kg/m(2)) (sex ratio=0.221, mean age 48.41±10.92 years; mean BMI 36.6±4.8 kg/m(2)). Genotyping was performed using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). Glucose, insulin, and lipids were measured. BMI and homeostasis model assessment of insulin resistance (HOMA-IR) were calculated.
RESULTS
The polymorphisms 276G/T, 639 C/T, 11391 G/A, 11374C/G, and +2019delA seem to contribute to obesity. In fact, adjusted odds ratios (ORs) of obesity associated with mutated genotypes of each polymorphism were, respectively: OR=0.64, P=0.039; OR=1.85, P=0.018; OR=1.68, P=0.044; OR=1.77, P=0.038; and OR=1.94, P=0.010). Mutated genotypes at 639 C/T were associated with higher waist circumference, BMI, and systolic and diastolic blood pressure. In addition, the 11391AA genotype was associated with increased BMI. Concerning 2019delA, the delAdelA genotype was associated with increased HOMA-IR and BMI, suggesting a possible effect of these single-nucleotide polymorphisms (SNPs) on insulin resistance parameters. Mutated genotypes at 276G/T were associated with lower serum insulin concentration and lower systolic and diastolic blood pressure. The other genotypes showed no association with metabolic syndrome parameters.
CONCLUSION
Adiponectin gene polymorphisms were associated with obesity and metabolic syndrome parameters in Tunisian volunteers.
背景
脂联素是一种由脂肪组织产生的血浆蛋白，具有胰岛素敏感性、抗炎和抗动脉粥样硬化特性。许多脂联素基因多态性已经被描述，它们与肥胖、代谢综合征和心血管疾病的关系存在争议。我们的目的是研究 8 种脂联素多态性（-1391G/A、-1377C/G、4522C/T、395G/A、276G/T、639C/T、45T/G 和 +2019delA）与代谢综合征参数以及突尼斯志愿者肥胖风险之间的关系。
方法
我们招募了 169 名非肥胖志愿者（性别比为 0.594，平均年龄 43.25±13.12 岁；平均体重指数（BMI）为 24.73±3.50kg/m²）和 160 名肥胖志愿者（BMI≥30kg/m²）（性别比为 0.221，平均年龄 48.41±10.92 岁；平均 BMI 为 36.6±4.8kg/m²）。使用聚合酶链反应限制片段长度多态性（PCR-RFLP）进行基因分型。测量血糖、胰岛素和脂质。计算 BMI 和稳态模型评估的胰岛素抵抗（HOMA-IR）。
结果
276G/T、639C/T、11391G/A、11374C/G 和 +2019delA 等多态性似乎与肥胖有关。事实上，每种多态性突变基因型与肥胖相关的校正优势比（OR）分别为：OR=0.64，P=0.039；OR=1.85，P=0.018；OR=1.68，P=0.044；OR=1.77，P=0.038；和 OR=1.94，P=0.010）。639C/T 的突变基因型与较高的腰围、BMI、收缩压和舒张压有关。此外，11391AA 基因型与 BMI 增加有关。关于 2019delA，delAdelA 基因型与升高的 HOMA-IR 和 BMI 有关，这表明这些单核苷酸多态性（SNP）可能对胰岛素抵抗参数有影响。276G/T 的突变基因型与较低的血清胰岛素浓度和较低的收缩压和舒张压有关。其他基因型与代谢综合征参数无关。
结论
脂联素基因多态性与突尼斯志愿者的肥胖和代谢综合征参数有关。
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