School of Mathematics and Physics, The University of Queensland, Brisbane 4072, Australia.
Opt Lett. 2009 Oct 1;34(19):2988-90. doi: 10.1364/OL.34.002988.
Differential interference contrast microscopy, imaging by interferometric superposition of two displaced beams passed through a transparent sample, is one of the most sophisticated methods in classical microscopy. Here we demonstrate a versatile electronically controlled variant using a liquid-crystal spatial light modulator that displays a diffractive optical element and steers the beam separation. The orientation and magnitude of the shear angle and the relative phase of the two interfering beams can all be varied at video rates. The technique is demonstrated by imaging polystyrene beads in immersion oil and a sample of red blood cells. The method expands the capabilities of previous implementations of differential interference contrast microscopy by its nonmechanical control over all imaging parameters.
微分干涉差显微镜是一种通过穿过透明样本的两个位移光束的干涉叠加来进行成像的技术，是经典显微镜中最复杂的方法之一。在这里，我们展示了一种使用液晶空间光调制器的多功能电子控制变体，该调制器可以显示衍射光学元件并控制光束分离。剪切角的方向和大小以及两束干涉光的相对相位都可以在视频速率下变化。该技术通过对浸油中的聚苯乙烯珠和红细胞样本进行成像来演示。该方法通过对所有成像参数进行非机械控制，扩展了以前的微分干涉差显微镜实现的功能。
