INSERM/UEVE UMR 861; I-STEM; AFM; Evry, France.
Organogenesis. 2009 Apr;5(2):62-6. doi: 10.4161/org.5.2.9091.
Recent studies have significantly improved our ability to investigate cell transplantation and study the physiology of transplanted cells in cardiac tissue. Several previous studies have shown that fully-immersed heart slices can be used for electrophysiological investigations. Additionally, ischemic heart slices induced by glucose and oxygen deprivation offer a useful tool to investigate mechanical integration and to measure forces of contraction of engrafted cells, at least for short term analysis. A recent and novel model of heart slices, prepared from rat and human tissues, can be maintained in culture for up to two months. This new heart slice model can be used for long term in vitro cell transplantation studies and for pharmacological evaluation. This review will focus on describing these models and demonstrating the use of organotypic heart slices as a novel tool for drugs for studying electrophysiology and developing cellular therapeutic approaches to alleviate cardiac tissue damage.
最近的研究显著提高了我们调查细胞移植和研究心脏组织中移植细胞生理学的能力。几项先前的研究表明，完全浸没的心脏切片可用于电生理研究。此外，通过葡萄糖和氧气剥夺诱导的缺血性心脏切片为研究机械整合和测量植入细胞的收缩力提供了有用的工具，至少对于短期分析而言。最近开发的一种新型大鼠和人组织来源的心脏切片模型可在培养中维持长达两个月。这种新型心脏切片模型可用于长期的体外细胞移植研究和药物评估。本综述将重点描述这些模型，并展示器官型心脏切片作为一种新工具，用于研究电生理学和开发细胞治疗方法以减轻心脏组织损伤的药物。
