Travers Andrew, Caserta Micaela, Churcher Mark, Hiriart Edwige, Di Mauro Ernesto
Fondation Pierre-Gilles de Gennes pour la Recherche, c/o LBPA, Ecole Normale Supérieure de Cachan, 61 Avenue de Président Wilson, 94235 Cachan Cedex, France.
Mol Biosyst. 2009 Dec;5(12):1582-92. doi: 10.1039/b907227f. Epub 2009 Sep 30.
The positioning of nucleosomes on the DNA of eukaryotic genomes is a major determinant of gene expression. In particular nucleosomes in close proximity to regulatory regions are often more precisely positioned in vivo than nucleosomes located elsewhere. In this article we compare data obtained from the most recent studies by a variety of techniques. We argue that the disparate conclusions in the literature could be a consequence of procedural differences sampling alternative arrays of nucleosomes on the same DNA sequence. Importantly, the ostensibly least invasive techniques identify differences between nucleosomes in the vicinity of transcription start sites in budding yeast and those positioned distally within the transcribed region.
核小体在真核生物基因组DNA上的定位是基因表达的主要决定因素。特别是，靠近调控区域的核小体在体内的定位通常比位于其他位置的核小体更为精确。在本文中，我们比较了通过各种技术从最新研究中获得的数据。我们认为，文献中不同的结论可能是由于程序差异导致在相同DNA序列上对核小体替代阵列进行采样的结果。重要的是，表面上侵入性最小的技术揭示了芽殖酵母转录起始位点附近的核小体与转录区域内远端定位的核小体之间的差异。
