耐甲氧西林和甲氧西林敏感金黄色葡萄球菌 398 型的短期微观进化和 PCR 检测。
Short term micro-evolution and PCR-detection of methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398.
机构信息
ErasmusMC, Department of Medical Microbiology and Infectious Diseases, University Medical Centre Rotterdam, Rotterdam, The Netherlands.
出版信息
Eur J Clin Microbiol Infect Dis. 2010 Jan;29(1):119-22. doi: 10.1007/s10096-009-0816-3. Epub 2009 Oct 2.
Micro-evolutionary analysis of 70 ST398 isolates by pulsed-field gel electrophoresis (PFGE) using Cfr9I revealed three sub-clones with abundant inter- and intra-sub-clone heterogeneity in spa- and SCCmec-types. In addition, we developed two specific PCRs for the detection of Staphylococcus aureus sequence type 398 (ST 398) isolates with 100% specificity and high sensitivity.
通过脉冲场凝胶电泳（PFGE）对 70 株 ST398 分离株进行微进化分析，发现三个亚克隆，在 spa 和 SCCmec 型中存在丰富的亚克隆间和亚克隆内异质性。此外，我们开发了两种用于检测金黄色葡萄球菌 398 型（ST398）分离株的特异性 PCR，具有 100%的特异性和高灵敏度。
Zotero 插件