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[氯化镉对葡萄根系线粒体特性及根系活力的影响]

[Effects of CdCl2 on grape root mitochondrial characteristics and root activity].

作者信息

Shao Xiao-Jie, Yang Hong-Qiang, Qiao Hai-Tao, Zhang Long, You Shu-Zhen

机构信息

State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an 271018, Shandong, China.

出版信息

Ying Yong Sheng Tai Xue Bao. 2009 Jun;20(6):1390-4.

Abstract

Taking the one-year cutting-seedlings of four grape cultivars (Kyoho, Muscat Hamburg, Long Yan, and Ze Xiang) as test materials, their root mitochondrial hydrogen peroxide (H2O2) content, membrane permeability transition pore (MPTP), membrane potential (Deltapsi), cytochrome C (Cyt c) content, and root activity were measured under effects of CdCl2. For all test grape cultivars, the root mitochondrial H2O2 content and MPTP increased, but the Deltapsi, Cyt c content, and root activity decreased after treated with 0.5 mmol CdCl2 x L(-1). The mitochondrial H2O2 content and Cyt c content were in the sequence of Kyoho > Ze Xiang > Muscat Hamburg > Long Yan, while the root activity, MPTP, and Deltapsi were in adverse, i.e., Long Yan > Muscat Hamburg > Ze Xiang > Kyoho. Among the test grape cultivars, the root activity of Kyoho was easier to be inhibited under Cd stress, while that of Long Yan was least affected by CdCl2.

摘要

以4个葡萄品种(巨峰、玫瑰香、龙眼和泽香)的1年生扦插苗为试验材料,测定了CdCl₂处理下其根系线粒体过氧化氢(H₂O₂)含量、膜通透性转换孔(MPTP)、膜电位(ΔΨ)、细胞色素C(Cyt c)含量及根系活力。对于所有供试葡萄品种,用0.5 mmol CdCl₂·L⁻¹处理后,根系线粒体H₂O₂含量和MPTP增加,而ΔΨ、Cyt c含量和根系活力降低。线粒体H₂O₂含量和Cyt c含量的顺序为巨峰>泽香>玫瑰香>龙眼,而根系活力、MPTP和ΔΨ则相反,即龙眼>玫瑰香>泽香>巨峰。在供试葡萄品种中,巨峰根系活力在Cd胁迫下较易受到抑制,而龙眼受CdCl₂的影响最小。

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