NanoBio Fusion Research Center, Korea Research Institute of Chemical Technology, Daejeon, Korea.
ACS Nano. 2009 Nov 24;3(11):3649-55. doi: 10.1021/nn900540a.
We herein demonstrate that Ni-decorated single-walled carbon nanotube field effect transistors (SWNT-FETs) combined with antibody fragments can be used as effective biosensing platforms. Nanoscales Ni particles 20 to 60 nm in diameter were formed on the sidewalls of SWNT-FETs using an electrochemical method. Carcinoembryonic antigen (CEA)-binding single chain variable fragments (scFvs) with a hexahistidine tag [(his)(6)] were synthesized using genetic engineering, and ordered immobilization of anti-CEA ScFvs on Ni nanoparticles was achieved by exploiting the specific interaction between hexahistidine and Ni. Whereas randomly oriented anti-CEA scFvs did not impart a noticeable change of conductance upon addition of CEA, a clear increase in conductance was observed using Ni-decorated SWNT-FETs functionalized with engineered scFvs.
我们在此证明，经过修饰的镍的单壁碳纳米管场效应晶体管（SWNT-FET）与抗体片段相结合，可以作为有效的生物传感平台。采用电化学方法在 SWNT-FET 的侧壁上形成了直径为 20 至 60nm 的纳米级 Ni 颗粒。利用遗传工程合成了带有六组氨酸标签[(his)6]的癌胚抗原（CEA）结合单链可变片段（scFv），通过六组氨酸和 Ni 之间的特异性相互作用，实现了对 Ni 纳米粒子上抗 CEA scFv 的有序固定。然而，当添加 CEA 时，随机取向的抗 CEA scFv 并没有引起明显的电导变化，而用工程 scFv 功能化的 Ni 修饰的 SWNT-FET 则观察到电导的明显增加。
