Simunović-Soskić Marica, Pezelj-Ribarić Sonja, Brumini Gordana, Glazar Irena, Grzić Renata, Miletić Ivana
Department of Prosthodontics, School of Dentistry, Medical Faculty, University of Rijeka, Croatia.
Photomed Laser Surg. 2010 Apr;28(2):189-93. doi: 10.1089/pho.2008.2420.
The aim of this study was to monitor therapeutic response by determining the level of proinflammatory cytokines TNF-alpha and IL-6 in whole unstimulated saliva in patients with denture stomatitis (DS), before and after laser phototherapy (LPT).
DS is an inflammatory condition that occurs in subjects who wear dentures, and it is a common oral mucosal lesion. A potential noninvasive treatment for DS patients is LPT.
A sample consisting of 40 consecutive subjects was selected on a voluntary basis from patients who presented for the diagnosis and treatment of DS at the Oral Medicine Unit of the Medical Faculty at the University of Rijeka. A clinical examination was performed according to the standard clinical criteria. Lesions described as palatal inflammation were diagnosed as Newton type II denture stomatitis. The patients were randomly assigned to either an experimental group (20 patients receiving real LPT) or a control group (20 patients receiving inactive/placebo laser treatment). In order to determine the salivary levels of TNF-alpha and IL-6, ELISA (Sigma Immunochemicals, St Louis, MO) was performed.
Following treatment with LPT for 4 wk, the levels of TNF-alpha and IL-6 decreased significantly (p < 0.001) and were significantly different from controls (p < 0.001).
The results of this study suggest that LPT may be an efficacious choice of therapy.
本研究旨在通过测定义齿性口炎（DS）患者在激光光疗（LPT）前后全唾液中促炎细胞因子肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）的水平来监测治疗反应。
DS是一种发生在佩戴义齿者中的炎症性疾病，是一种常见的口腔黏膜病变。LPT是一种针对DS患者的潜在非侵入性治疗方法。
从里耶卡大学医学院口腔医学科前来诊断和治疗DS的患者中自愿选取40名连续受试者作为样本。根据标准临床标准进行临床检查。将描述为腭部炎症的病变诊断为牛顿II型义齿性口炎。患者被随机分为实验组（20例接受真正的LPT）或对照组（20例接受无效/安慰剂激光治疗）。为了测定唾液中TNF-α和IL-6的水平，采用酶联免疫吸附测定法（ELISA，Sigma免疫化学公司，密苏里州圣路易斯）。
LPT治疗4周后，TNF-α和IL-6水平显著降低（p<0.001），且与对照组有显著差异（p<0.001）。
本研究结果表明LPT可能是一种有效的治疗选择。
