Medicine University of Tunis, Tunisia.
Clin Exp Rheumatol. 2009 Mar-Apr;27(2 Suppl 53):S52-7.
Matrix metalloproteinases (MMP-) are involved in leukocyte invasion into the central nervous system (CNS) during inflammation. In a retrospective cohort study of 18 neuro-BD patients, CSF samples were studied for MMP-9, TIMP-1 and cell characteristics in neuro-BD patients compared to 12 Headache attributed to BD (HaBD) patients, 15 multiple sclerosis (MS) and 20 Non-inflammatory Neurological Disease (NIND) patients. Concentrations of MMP-9 and TIMP-1 were measured in CSF by using an enzyme-linked immunosorbent assay (ELISA). The MMP-9/TIMP-1 ratio was significantly increased in neuro-BD group (mean +/- SD: 0.145+/-0.045) compared to (HaBD) (0.065+/-0.029; p=0.0001) and NIND patients (0.070+/-0.031; p=0.0001). No significant differences were observed between neuro-BD and MS patients. A significant correlation was observed between CSF-PMN cells and MMP-9 in neuro-BD patients (r=0.714; p=0.0009), indicating probably that PMN cells were in part the source of MMP-9. A significantly positive correlation was also observed between MMP-9 and CSF-mononuclear cells in neuro-BD patients (r=0.623; p=0.0012). This is the first study to evaluate the expression of matrix metalloproteinase-9 and tissue inhibitors of metalloproteinase-1 in cerebrospinal fluid of neuro-BD patients. It demonstrates increased matrix metalloproteinase-9/tissue inhibitors of metalloproteinase-1 ratio. The results suggested that MMPs released in the CSF may be involved in the pathogenesis of neuro-BD by promoting local damage, similarly as suspected in other inflammatory diseases.
基质金属蛋白酶（MMP-）参与炎症期间白细胞浸润中枢神经系统（CNS）。在一项对 18 例神经 Behcet 病（neuro-Behcet disease，neuro-BD）患者的回顾性队列研究中，与 12 例 Behcet 病头痛归因于（Headache attributed to BD，HaBD）患者、15 例多发性硬化症（multiple sclerosis，MS）和 20 例非炎症性神经疾病（Non-inflammatory Neurological Disease，NIND）患者相比，研究了脑脊液中 MMP-9、TIMP-1 和细胞特征。通过酶联免疫吸附测定（enzyme-linked immunosorbent assay，ELISA）测量 CSF 中 MMP-9 和 TIMP-1 的浓度。与 HaBD（0.065+/-0.029；p=0.0001）和 NIND 患者（0.070+/-0.031；p=0.0001）相比，神经 BD 组的 MMP-9/TIMP-1 比值显著升高（平均值 +/- 标准差：0.145+/-0.045）。未观察到神经 BD 与 MS 患者之间的显著差异。在神经 BD 患者中，CSF-PMN 细胞与 MMP-9 之间观察到显著相关性（r=0.714；p=0.0009），表明 PMN 细胞可能部分是 MMP-9 的来源。神经 BD 患者的 MMP-9 与 CSF 单核细胞之间也观察到显著正相关（r=0.623；p=0.0012）。这是第一项评估神经 BD 患者脑脊液中基质金属蛋白酶-9 和金属蛋白酶组织抑制剂-1 表达的研究。它显示出 MMP-9/金属蛋白酶组织抑制剂-1 比值增加。结果表明，CSF 中释放的 MMPs 可能通过促进局部损伤而参与神经 BD 的发病机制，这与其他炎症性疾病中的推测类似。
