Hanin-Avraham Nirit, Fuhrman Bianca, Mech-Dorosz Agnieszka, Kolusheva Sofiya, Porgador Angel, Aviram Michael, Jelinek Raz
Department of Chemistry, Ben Gurion University of the Negev, Beer Sheva 84105, Israel.
Biochim Biophys Acta. 2009 Nov;1788(11):2436-43. doi: 10.1016/j.bbamem.2009.09.013. Epub 2009 Sep 29.
Changes in the abundance and properties of blood lipoproteins are generally considered major causes for varied pathological conditions and diseases. Using novel chromatic biomimetic vesicle and cell assays, we present here for the first time evidence for significant changes in lipoproteins' interactions with artificial membranes. Specifically, we demonstrate significant differences in membrane binding between lipoproteins (both low-density lipoprotein [LDL] and high-density lipoprotein [HDL]) harvested from diabetic patients vs. healthy controls as well as between oxidized and native lipoproteins. The chromatic assays, complemented by biophysical techniques and electron microscopy, point to significant reduction of surface membrane binding of the lipoproteins as a consequence of diabetes or oxidation. Overall, our results indicate that the substantial modulation of membrane interactions revealed by the chromatic assays may be used as a new and potentially powerful marker for screening and prediction of diseases associated with oxidative stress.
血液脂蛋白丰度和特性的变化通常被认为是多种病理状况和疾病的主要原因。我们使用新型彩色仿生囊泡和细胞检测方法，首次在此展示了脂蛋白与人工膜相互作用发生显著变化的证据。具体而言，我们证明了从糖尿病患者与健康对照者体内获取的脂蛋白（低密度脂蛋白[LDL]和高密度脂蛋白[HDL]）之间以及氧化型和天然脂蛋白之间在膜结合上存在显著差异。这些彩色检测方法，辅以生物物理技术和电子显微镜，表明糖尿病或氧化会导致脂蛋白表面膜结合显著减少。总体而言，我们的结果表明，彩色检测所揭示的膜相互作用的实质性调节可能用作筛查和预测与氧化应激相关疾病的一种新的且潜在强大的标志物。
