八聚体结合因子Oct6：cDNA克隆及在早期胚胎细胞中的表达

The octamer binding factor Oct6: cDNA cloning and expression in early embryonic cells.

作者信息

Meijer D, Graus A, Kraay R, Langeveld A, Mulder M P, Grosveld G

机构信息

MGC Department of Cell Biology and Genetics, Erasmus University, Rotterdam, The Netherlands.

出版信息

Nucleic Acids Res. 1990 Dec 25;18(24):7357-65. doi: 10.1093/nar/18.24.7357.

We have cloned a cDNA encoding a novel octamer binding factor Oct6 that is expressed in undifferentiated ES cells. Expression of the Oct6 gene is downregulated upon differentiation of these cells by aggregate formation. Furthermore the gene is transiently up regulated during retinoic acid induced differentiation of P19 EC cells, reaching maximum levels of expression one day after RA addition. Sequence analysis of the cDNA encoding the Oct6 protein indicated that the Oct6 gene is a member of the POU-HOMEO domain gene family. The gene expresses a 3 kb mRNA encoding a 449 amino acid protein with an apparent molecular weight of 45 kD. The sequence of the Oct6 POU domain is identical to that of the rat SCIP (Tst-1) gene. The Oct6 expression pattern suggests a role for this DNA binding protein in neurogenesis as well as early embryogenesis.

我们已经克隆了一个编码新型八聚体结合因子Oct6的cDNA，该因子在未分化的胚胎干细胞中表达。通过聚集体形成使这些细胞分化时，Oct6基因的表达下调。此外，在视黄酸诱导P19胚胎癌细胞分化过程中，该基因会短暂上调，在添加视黄酸一天后达到最大表达水平。对编码Oct6蛋白的cDNA进行序列分析表明，Oct6基因是POU同源结构域基因家族的成员。该基因表达一种3kb的mRNA，编码一个449个氨基酸的蛋白质，表观分子量为45kD。Oct6 POU结构域的序列与大鼠SCIP（Tst-1）基因的序列相同。Oct6的表达模式表明这种DNA结合蛋白在神经发生以及早期胚胎发生中发挥作用。