Lichtsteiner S, Wuarin J, Schibler U
Department of Molecular Biology, University of Geneva, Switzerland.
Cell. 1987 Dec 24;51(6):963-73. doi: 10.1016/0092-8674(87)90583-6.
The promoter of the mouse albumin gene contains at least six binding sites for specific DNA-binding proteins (A to F). Four of these sites (A, D, E, and F) can be occupied by transcription factors that are considerably enriched in liver nuclei, as compared to spleen or brain nuclei. These factors consist of a heat-stable protein that fills sites A, D, and F, and a member of a family of nuclear factor I (NF-I) related proteins that occupies site E. Site C binds a protein that is equally abundant in liver, brain, and spleen nuclei. Occupancy of this site and the binding of the heat-stable factor to the immediately adjacent site D appear to be mutually exclusive. However, both of these competing binding sites are required for maximal in vitro transcription.
小鼠白蛋白基因的启动子含有至少六个特定DNA结合蛋白（A至F）的结合位点。与脾或脑细胞核相比，这些位点中的四个（A、D、E和F）可被在肝细胞核中大量富集的转录因子占据。这些因子包括一种填充位点A、D和F的热稳定蛋白，以及占据位点E的核因子I（NF-I）相关蛋白家族的一个成员。位点C结合一种在肝、脑和脾细胞核中含量相同的蛋白。该位点的占据以及热稳定因子与紧邻的位点D的结合似乎是相互排斥的。然而，这两个相互竞争的结合位点对于最大程度的体外转录都是必需的。
