Henry M M, Moore J N, Feldman E B, Fischer J K, Russell B
Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens 30602.
Circ Shock. 1990 Nov;32(3):173-88.
To investigate the effects of an omega-3 fatty acid-enriched ration on the in vitro response of equine monocytes to endotoxin, an 8-week feeding trial was conducted in which linseed oil served as the source of the omega-3 fatty acid, alpha-linolenic acid. One group of horses was fed a control pelleted ration and the other group was fed an 8% linseed oil-enriched pelleted ration. After 8 weeks of feeding, monocytes were isolated and incubated in the presence of Escherichia coli O55:B5 endotoxin for 6 hr. After 8 weeks on the rations, the mean procoagulant activity and thromboxane B2 production by endotoxin-stimulated monocytes from horses consuming the linseed oil ration decreased by 51% and 71%, respectively, compared with cells from horses consuming the control ration. There was no difference in monocyte synthesis of 12-hydroxyeicosatetraenoic acid or leukotriene B4 between groups. Fatty acid analysis of membrane phospholipids revealed a decrease in the omega-6:omega-3 ratio in monocytes from horses consuming the linseed oil ration. These data suggest that dietary supplementation with alpha-linolenic acid may modify the response to endotoxin by reducing the synthesis of potentially harmful cellular mediators.
为研究富含ω-3脂肪酸的日粮对马单核细胞体外对内毒素反应的影响，进行了一项为期8周的饲养试验，其中以亚麻籽油作为ω-3脂肪酸α-亚麻酸的来源。一组马饲喂对照颗粒日粮，另一组饲喂添加8%亚麻籽油的颗粒日粮。饲喂8周后，分离单核细胞，并在大肠杆菌O55:B5内毒素存在的情况下孵育6小时。在日粮饲喂8周后，与食用对照日粮的马的细胞相比，食用亚麻籽日粮的马的内毒素刺激单核细胞的平均促凝活性和血栓素B2生成分别降低了51%和71%。两组之间单核细胞合成12-羟基二十碳四烯酸或白三烯B4没有差异。膜磷脂的脂肪酸分析显示，食用亚麻籽日粮的马的单核细胞中ω-6:ω-3比例降低。这些数据表明，日粮中补充α-亚麻酸可能通过减少潜在有害细胞介质的合成来改变对内毒素的反应。
