Bowness J M
Atherosclerosis. 1977 Jun;27(2):221-5. doi: 10.1016/0021-9150(77)90059-4.
The interaction of low density lipoprotein (LDL) with solubilized subunits of two purified noncollagenous glycoproteins (A and G) of cartilage matrix have been studied in solution using Sepharose chromatography and at the cell surface using fibroblast monolayers. In the presence of A or G, [125I]-LDL forms aggregates of varying size. After pre-incubation with A or G, under conditions which form highly aggregrated complexes, there is an increase in the binding of LDL to fibroblasts. Mixing with A under conditions which form smaller complexes produced no increase in binding to fibroblasts. It is suggested that interactions of this type may be involved in the pathogenesis of atherosclerosis.
利用琼脂糖凝胶色谱法在溶液中以及利用成纤维细胞单层在细胞表面研究了低密度脂蛋白（LDL）与软骨基质的两种纯化的非胶原蛋白糖蛋白（A和G）的可溶亚基之间的相互作用。在存在A或G的情况下，[125I]-LDL形成大小各异的聚集体。在与A或G预孵育后，在形成高度聚集复合物的条件下，LDL与成纤维细胞的结合增加。在形成较小复合物的条件下与A混合，并未导致与成纤维细胞的结合增加。提示这类相互作用可能参与动脉粥样硬化的发病机制。
