Kogata Naoko, Tribe Rachel M, Fässler Reinhard, Way Michael, Adams Ralf H
Vascular Development Laboratory, UK London Research Institute, London WC2A 3PX, United Kingdom.
Genes Dev. 2009 Oct 1;23(19):2278-83. doi: 10.1101/gad.535409.
Vascular smooth muscle cells (VSMCs) form contractile layers around larger blood vessels in a process that is essential for the formation of a fully functional vasculature. Here, we show that integrin-linked kinase (ILK) is required for the formation of a unitary layer of aligned VSMCs around arterioles and the regulation of blood vessel constriction in mice. In the absence of ILK, activated Rho/ROCK signaling induces the elevated phosphorylation of myosin light chain leading to abnormally enhanced VSMC contraction in vitro and in vivo. Our findings identify ILK as a key component regulating vascular wall formation by negatively modulating VSMC contractility.
血管平滑肌细胞（VSMCs）在较大血管周围形成收缩层，这一过程对于形成功能完备的脉管系统至关重要。在此，我们表明整合素连接激酶（ILK）对于小鼠小动脉周围排列整齐的VSMCs单层的形成以及血管收缩的调节是必需的。在缺乏ILK的情况下，激活的Rho/ROCK信号传导会导致肌球蛋白轻链磷酸化升高，从而在体外和体内导致VSMC收缩异常增强。我们的研究结果表明ILK是通过负向调节VSMC收缩性来调节血管壁形成的关键成分。
