Shults C W, Hashimoto R, Brady R M, Gage F H
Neurology Service (127), VA Medical Center, San Diego, CA 92161.
Neuroscience. 1990;38(2):427-36. doi: 10.1016/0306-4522(90)90039-7.
Studies were performed to examine the relation of dopaminergic cells and radial glia in the developing mesencephalon of the rat at ages E12-E20. Dopaminergic cells were immunolabelled with an antiserum which recognizes tyrosine hydroxylase, and radial glia were immunolabelled with a monoclonal antibody which recognizes vimentin. The vimentin-immunoreactive fibres of radial glia were noted at E12. At E12, and more clearly at later time points, the radial glia extended from the aqueduct to the pial surface, and this pattern persisted throughout the prenatal period. Tyrosine hydroxylase-immunoreactive cells were located along the ventral surface of the mesencephalon at age E13. At age E15, E16, and E18 the tyrosine hydroxylase-immunoreactive cells were present from the aqueduct to the ventral pial surface of the mesencephalon and were aligned along radial glia. Our study suggests that radial glia provide paths for migration of dopaminergic cells in the mantle layer from E15 to E18 of the developing mesencephalon. It also suggests that some dopaminergic cells between E15 and E18 may express tyrosine hydroxylase during their migration through the mantle layer and prior to reaching the location they occupy in the adult brain.
开展了多项研究，以检测大鼠胚胎第12至20天发育中的中脑中多巴胺能细胞与放射状胶质细胞之间的关系。用识别酪氨酸羟化酶的抗血清对多巴胺能细胞进行免疫标记，用识别波形蛋白的单克隆抗体对放射状胶质细胞进行免疫标记。在胚胎第12天观察到放射状胶质细胞的波形蛋白免疫反应性纤维。在胚胎第12天以及之后更明显的时间点，放射状胶质细胞从导水管延伸至软膜表面，且这种模式在整个孕期持续存在。在胚胎第13天，酪氨酸羟化酶免疫反应性细胞位于中脑腹侧表面。在胚胎第15、16和18天，酪氨酸羟化酶免疫反应性细胞从中脑导水管至腹侧软膜表面均有分布，并沿放射状胶质细胞排列。我们的研究表明，放射状胶质细胞为发育中脑的套层中多巴胺能细胞在胚胎第15至18天的迁移提供路径。这也表明，胚胎第15至18天的一些多巴胺能细胞在通过套层迁移期间以及到达其在成体脑中占据的位置之前可能表达酪氨酸羟化酶。
