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秀丽隐杆线虫减数分裂重组分析。

Analysis of meiotic recombination in Caenorhabditis elegans.

作者信息

Hillers Kenneth J, Villeneuve Anne M

机构信息

Biological Sciences, California Polytechnic State University, San Luis Obispo, CA, USA.

出版信息

Methods Mol Biol. 2009;557:77-97. doi: 10.1007/978-1-59745-527-5_7.

DOI:10.1007/978-1-59745-527-5_7
PMID:19799178
Abstract

Caenorhabditis elegans is an important experimental organism for the study of recombination during meiosis. A variety of techniques have been developed for the measurement of meiotic recombination in C. elegans, ranging from traditional genetic measures to direct cytological determination of chiasma frequency. Here, we provide methods for some of the varied approaches used for the study of meiotic recombination in these tiny but powerful worms.

摘要

秀丽隐杆线虫是研究减数分裂过程中重组的重要实验生物。已经开发出了多种技术来测量秀丽隐杆线虫中的减数分裂重组,从传统的遗传学方法到直接细胞学测定交叉频率。在这里,我们提供了一些用于研究这些微小但强大的线虫减数分裂重组的不同方法。

相似文献

1
Analysis of meiotic recombination in Caenorhabditis elegans.秀丽隐杆线虫减数分裂重组分析。
Methods Mol Biol. 2009;557:77-97. doi: 10.1007/978-1-59745-527-5_7.
2
SNP-based mapping of crossover recombination in Caenorhabditis elegans.基于单核苷酸多态性(SNP)的秀丽隐杆线虫交叉重组图谱分析
Methods Mol Biol. 2011;745:207-22. doi: 10.1007/978-1-61779-129-1_13.
3
The role of chromosome ends during meiosis in Caenorhabditis elegans.秀丽隐杆线虫减数分裂过程中染色体末端的作用。
Bioessays. 1996 Jun;18(6):447-52. doi: 10.1002/bies.950180606.
4
Chromosome structure and homologous chromosome association during meiotic prophase in Caenorhabditis elegans.秀丽隐杆线虫减数分裂前期的染色体结构和同源染色体联会
Methods Mol Biol. 2011;745:549-62. doi: 10.1007/978-1-61779-129-1_32.
5
Selection at linked sites in the partial selfer Caenorhabditis elegans.秀丽隐杆线虫部分自体受精者中连锁位点的选择。
Mol Biol Evol. 2003 May;20(5):665-73. doi: 10.1093/molbev/msg072. Epub 2003 Apr 2.
6
Meiosis: checking chromosomes pair up properly.减数分裂:检查染色体是否正确配对。
Curr Biol. 2006 Apr 4;16(7):R249-51. doi: 10.1016/j.cub.2006.03.002.
7
Rapid single nucleotide polymorphism mapping in C. elegans.秀丽隐杆线虫中的快速单核苷酸多态性定位
BMC Genomics. 2005 Sep 12;6:118. doi: 10.1186/1471-2164-6-118.
8
Cohesin SMC1 beta is required for meiotic chromosome dynamics, sister chromatid cohesion and DNA recombination.黏连蛋白SMC1β是减数分裂染色体动态变化、姐妹染色单体黏连和DNA重组所必需的。
Nat Cell Biol. 2004 Jun;6(6):555-62. doi: 10.1038/ncb1135. Epub 2004 May 16.
9
Methods for analyzing checkpoint responses in Caenorhabditis elegans.秀丽隐杆线虫中检查点反应的分析方法。
Methods Mol Biol. 2004;280:257-74. doi: 10.1385/1-59259-788-2:257.
10
Characterization of a Caenorhabditis elegans recA-like gene Ce-rdh-1 involved in meiotic recombination.参与减数分裂重组的秀丽隐杆线虫recA样基因Ce-rdh-1的特性分析
DNA Res. 1998 Dec 31;5(6):373-7. doi: 10.1093/dnares/5.6.373.

引用本文的文献

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C. elegans Gonad Dissection and Freeze Crack for Immunofluorescence and DAPI Staining.秀丽隐杆线虫性腺解剖和用于免疫荧光和 DAPI 染色的冷冻破解。
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2
Poly(ADP-ribose) glycohydrolase coordinates meiotic DNA double-strand break induction and repair independent of its catalytic activity.聚(ADP-核糖)糖水解酶通过其催化活性以外的机制协调减数分裂 DNA 双链断裂的诱导和修复。
Nat Commun. 2020 Sep 25;11(1):4869. doi: 10.1038/s41467-020-18693-1.
3
The conserved LEM-3/Ankle1 nuclease is involved in the combinatorial regulation of meiotic recombination repair and chromosome segregation in Caenorhabditis elegans.
保守的 LEM-3/Ankle1 核酸酶参与了秀丽隐杆线虫减数分裂重组修复和染色体分离的组合调控。
PLoS Genet. 2018 Jun 7;14(6):e1007453. doi: 10.1371/journal.pgen.1007453. eCollection 2018 Jun.
4
The SMC-5/6 Complex and the HIM-6 (BLM) Helicase Synergistically Promote Meiotic Recombination Intermediate Processing and Chromosome Maturation during Caenorhabditis elegans Meiosis.SMC-5/6复合物与HIM-6(BLM)解旋酶协同促进秀丽隐杆线虫减数分裂过程中的减数分裂重组中间体加工和染色体成熟。
PLoS Genet. 2016 Mar 24;12(3):e1005872. doi: 10.1371/journal.pgen.1005872. eCollection 2016 Mar.
5
Meiosis.减数分裂
WormBook. 2017 May 4;2017:1-43. doi: 10.1895/wormbook.1.178.1.
6
Proteomic identification of germline proteins in Caenorhabditis elegans.秀丽隐杆线虫种系蛋白的蛋白质组学鉴定
Worm. 2015 Feb 9;4(1):e1008903. doi: 10.1080/21624054.2015.1008903. eCollection 2015 Jan-Mar.
7
Combinatorial regulation of meiotic holliday junction resolution in C. elegans by HIM-6 (BLM) helicase, SLX-4, and the SLX-1, MUS-81 and XPF-1 nucleases.在秀丽隐杆线虫中,由 HIM-6(BLM)解旋酶、SLX-4 和 SLX-1、MUS-81 和 XPF-1 核酸内切酶进行的减数分裂 Holliday 连接体的组合调控。
PLoS Genet. 2013;9(7):e1003591. doi: 10.1371/journal.pgen.1003591. Epub 2013 Jul 18.
8
Caenorhabditis elegans chromatin-associated proteins SET-2 and ASH-2 are differentially required for histone H3 Lys 4 methylation in embryos and adult germ cells.秀丽隐杆线虫染色质相关蛋白 SET-2 和 ASH-2 在胚胎和成年生殖细胞中对组蛋白 H3 Lys 4 甲基化的需求不同。
Proc Natl Acad Sci U S A. 2011 May 17;108(20):8305-10. doi: 10.1073/pnas.1019290108. Epub 2011 Apr 28.
9
An asymmetric chromosome pair undergoes synaptic adjustment and crossover redistribution during Caenorhabditis elegans meiosis: implications for sex chromosome evolution.在秀丽隐杆线虫减数分裂过程中,一对非同源染色体通过联会调整和交叉重分配:对性染色体进化的启示。
Genetics. 2011 Mar;187(3):685-99. doi: 10.1534/genetics.110.124958. Epub 2011 Jan 6.