秀丽隐杆线虫减数分裂重组分析。

Analysis of meiotic recombination in Caenorhabditis elegans.

作者信息

Hillers Kenneth J, Villeneuve Anne M

机构信息

Biological Sciences, California Polytechnic State University, San Luis Obispo, CA, USA.

出版信息

Methods Mol Biol. 2009;557:77-97. doi: 10.1007/978-1-59745-527-5_7.

DOI:10.1007/978-1-59745-527-5_7

PMID:19799178

Abstract

Caenorhabditis elegans is an important experimental organism for the study of recombination during meiosis. A variety of techniques have been developed for the measurement of meiotic recombination in C. elegans, ranging from traditional genetic measures to direct cytological determination of chiasma frequency. Here, we provide methods for some of the varied approaches used for the study of meiotic recombination in these tiny but powerful worms.

摘要

秀丽隐杆线虫是研究减数分裂过程中重组的重要实验生物。已经开发出了多种技术来测量秀丽隐杆线虫中的减数分裂重组，从传统的遗传学方法到直接细胞学测定交叉频率。在这里，我们提供了一些用于研究这些微小但强大的线虫减数分裂重组的不同方法。