Santín Mónica, Vecino Jesús A Cortés, Fayer Ronald
Environmental Microbial and Food Safety Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, 10300 Baltimore Avenue, Beltsville, Maryland 20705, USA.
J Parasitol. 2010 Feb;96(1):157-61. doi: 10.1645/GE-2184.1.
This is the first report of Enterocytozoon bieneusi in an equid species. Feces from 195 horses from 4 locations in Colombia were examined for E. bieneusi by polymerase chain reaction. Of these, 21 horses (10.8%) were found positive for E. bieneusi . The prevalence of E. bieneusi in horses <1 yr of age was significantly higher (23.7%) than in horses >1 yr of age (2.5%). No significant differences in prevalence were observed between male (13.7%) and female horses (9%). Sequencing of the internal transcribed spacer region of the SSUrRNA locus identified 3 genotypes. Two genotypes appear to be unique to horses and were named Horse 1 and Horse 2. A third genotype, identified as genotype D, was detected in 4 horses. This genotype, previously reported to infect humans, beaver, cattle, dogs, falcons, foxes, macaques, muskrats, pigs, and raccoons, is the most ubiquitous of the E. bieneusi zoonotic genotypes. Our findings indicate that E. bieneusi from horses can be a potential source of infection for humans.
这是关于毕氏肠微孢子虫在马属动物中的首次报道。通过聚合酶链反应对来自哥伦比亚4个地点的195匹马的粪便进行了毕氏肠微孢子虫检测。其中，21匹马（10.8%）被检测出毕氏肠微孢子虫呈阳性。1岁以下马匹中毕氏肠微孢子虫的感染率（23.7%）显著高于1岁以上马匹（2.5%）。公马（13.7%）和母马（9%）之间的感染率无显著差异。对SSUrRNA基因座的内部转录间隔区进行测序鉴定出3种基因型。其中两种基因型似乎是马所特有的，分别命名为马1型和马2型。在4匹马中检测到第三种基因型，鉴定为D型。该基因型先前报道可感染人类、海狸、牛、狗、猎鹰、狐狸、猕猴、麝鼠、猪和浣熊，是毕氏肠微孢子虫人畜共患基因型中分布最广泛的。我们的研究结果表明，来自马的毕氏肠微孢子虫可能是人类潜在的感染源。
