Lee Hyunsang, Yoshizawa Susumu, Kogure Kazuhiro, Kim Hyun Soo, Yoon Jaewoo
College of Pharmacy, Keimyung University, 1095 Dalgubeoldaero, Dalseo-Gu, Daegu, 704-701, Republic of Korea.
Curr Microbiol. 2015 Apr;70(4):514-9. doi: 10.1007/s00284-014-0750-1. Epub 2014 Dec 7.
A strictly aerobic, Gram-negative, beige-pigmented, short-rod-shaped, non-motile and chemoheterotrophic bacteria, designated K2-48(T) was isolated from seawater collected in the Western North Pacific Ocean near Japan. Preliminary analysis based on the 16S rRNA gene sequence revealed that the novel isolate was affiliated with the family Oceanospirillaceae within the class Gammaproteobacteria and that it showed the highest sequence similarity (93.7 %) to Neptunomonas qingdaonensis P10-2-4(T). The strain could be differentiated phenotypically from recognized members of the family Oceanospirillaceae. The major fatty acids of strain K2-48(T) were identified as summed feature 3 (C16:1 ω7c and/or iso-C15:0 2-OH) and C16:0 as defined by the MIDI system. The DNA G+C content was determined to be 43.2 mol%, the major respiratory quinone was identified as ubiquinone 9 and a polar lipid profile was present consisting of phosphatidylethanolamine, a phosphatidylglycerol and an unidentified phospolipid. On the basis of polyphasic taxonomic studies, it was concluded that strain K2-48(T) represents a novel genus sp. We propose the name Pelagitalea pacifica gen. nov., sp. nov. for this strain; its type strain is K2-48(T) (=KCCM 90119(T)).
从日本附近的北太平洋西部采集的海水中分离出一株严格需氧、革兰氏阴性、米色色素、短杆状、不运动且化能异养的细菌，命名为K2-48(T)。基于16S rRNA基因序列的初步分析表明，该新分离株属于γ-变形菌纲中的海螺旋菌科，与青岛海单胞菌P10-2-4(T)的序列相似性最高（93.7%）。该菌株在表型上可与海螺旋菌科已确认的成员区分开来。根据MIDI系统的定义，菌株K2-48(T)的主要脂肪酸被鉴定为总和特征3（C16:1 ω7c和/或异-C15:0 2-OH）和C16:0。DNA G+C含量测定为43.2 mol%，主要呼吸醌被鉴定为泛醌9，并且存在由磷脂酰乙醇胺、磷脂酰甘油和一种未鉴定的磷脂组成的极性脂质谱。基于多相分类研究，得出结论：菌株K2-48(T)代表一个新的属种。我们为此菌株提议命名为太平洋远洋菌属（Pelagitalea pacifica），新属，新种；其模式菌株为K2-48(T)（=KCCM 90119(T)）。
