Will Torsten, Kornemann Sarah R, Furch Alexandra C U, Tjallingii W Fred, van Bel Aart J E
Plant Cell Biology Research Group, Department of General Botany, Justus-Liebig-University, Senckenbergstrasse 17-21, D-35390 Giessen, Germany.
J Exp Biol. 2009 Oct;212(Pt 20):3305-12. doi: 10.1242/jeb.028514.
Ca2+-binding proteins in the watery saliva of Megoura viciae counteract Ca2+-dependent occlusion of sieve plates in Vicia faba and so prevent the shut-down of food supply in response to stylet penetration. The question arises whether this interaction between aphid saliva and sieve-element proteins is a universal phenomenon as inferred by the coincidence between sieve-tube occlusion and salivation. For this purpose, leaf tips were burnt in a number of plant species from four different families to induce remote sieve-plate occlusion. Resultant sieve-plate occlusion in these plant species was counteracted by an abrupt switch of aphid behaviour. Each of the seven aphid species tested interrupted its feeding behaviour and started secreting watery saliva. The protein composition of watery saliva appeared strikingly different between aphid species with less than 50% overlap. Secretion of watery saliva seems to be a universal means to suppress sieve-plate occlusion, although the protein composition of watery saliva seems to diverge between species.
豌豆蚜水样唾液中的钙结合蛋白可抵消蚕豆筛板的钙依赖性堵塞，从而防止因口针穿刺而导致食物供应中断。问题在于，正如筛管堵塞与唾液分泌同时发生所推断的那样，蚜虫唾液与筛管分子蛋白之间的这种相互作用是否是一种普遍现象。为此，对来自四个不同科的多种植物的叶尖进行灼烧，以诱导远距离筛板堵塞。这些植物物种中由此产生的筛板堵塞被蚜虫行为的突然转变所抵消。所测试的七种蚜虫中的每一种都中断了取食行为并开始分泌水样唾液。不同蚜虫物种水样唾液的蛋白质组成差异显著，重叠率不到50%。分泌水样唾液似乎是抑制筛板堵塞的普遍手段，尽管不同物种水样唾液的蛋白质组成似乎有所不同。
