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Biogeography and phylogeny of the NOR5/OM60 clade of Gammaproteobacteria.γ-变形菌纲NOR5/OM60进化枝的生物地理学与系统发育
Syst Appl Microbiol. 2009 Apr;32(2):124-39. doi: 10.1016/j.syapm.2008.12.001.
2
The genome of Heliobacterium modesticaldum, a phototrophic representative of the Firmicutes containing the simplest photosynthetic apparatus.适度嗜盐栖热放线菌的基因组，它是厚壁菌门中具有最简单光合装置的光合代表菌。
J Bacteriol. 2008 Jul;190(13):4687-96. doi: 10.1128/JB.00299-08. Epub 2008 Apr 25.
3
Candidatus Chloracidobacterium thermophilum: an aerobic phototrophic Acidobacterium.嗜热嗜酸栖热菌：一种好氧光合嗜酸菌。
Science. 2007 Jul 27;317(5837):523-6. doi: 10.1126/science.1143236.
4
Chlorophyll biosynthesis in bacteria: the origins of structural and functional diversity.细菌中的叶绿素生物合成：结构与功能多样性的起源
Annu Rev Microbiol. 2007;61:113-29. doi: 10.1146/annurev.micro.61.080706.093242.
5
Assessing diversity and biogeography of aerobic anoxygenic phototrophic bacteria in surface waters of the Atlantic and Pacific Oceans using the Global Ocean Sampling expedition metagenomes.利用全球海洋采样探险宏基因组评估大西洋和太平洋表层水中需氧不产氧光合细菌的多样性和生物地理学。
Environ Microbiol. 2007 Jun;9(6):1464-75. doi: 10.1111/j.1462-2920.2007.01265.x.
6
Communities of green sulfur bacteria in marine and saline habitats analyzed by gene sequences of 16S rRNA and Fenna-Matthews-Olson protein.通过16S rRNA和费纳-马修斯-奥尔森蛋白的基因序列分析海洋和盐生栖息地中的绿色硫细菌群落。
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7
Hoeflea phototrophica sp. nov., a novel marine aerobic alphaproteobacterium that forms bacteriochlorophyll a.光养霍夫勒氏菌新种，一种能形成细菌叶绿素a的新型海洋需氧α-变形菌。
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8
Novel primers reveal wider diversity among marine aerobic anoxygenic phototrophs.新型引物揭示了海洋需氧不产氧光合细菌之间更广泛的多样性。
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Dinoroseobacter shibae gen. nov., sp. nov., a new aerobic phototrophic bacterium isolated from dinoflagellates.石滨玫瑰杆菌属新种，一种从甲藻中分离出的新型需氧光合细菌。
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Prevalence of the Chloroflexi-related SAR202 bacterioplankton cluster throughout the mesopelagic zone and deep ocean.与绿弯菌门相关的SAR202浮游细菌群落在中层带和深海中的分布情况。
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基于 BchY 的简并引物可在单个 PCR 中靶向所有类型的厌氧光合细菌。

BchY-based degenerate primers target all types of anoxygenic photosynthetic bacteria in a single PCR.

机构信息

National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA.

出版信息

Appl Environ Microbiol. 2009 Dec;75(23):7556-9. doi: 10.1128/AEM.01014-09. Epub 2009 Oct 2.

DOI:10.1128/AEM.01014-09
PMID:19801482
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2786397/
Abstract

To detect anoxygenic bacteria containing either type 1 or type 2 photosynthetic reaction centers in a single PCR, we designed a degenerate primer set based on the bchY gene. The new primers were validated in silico using the GenBank nucleotide database as well as by PCR on pure strains and environmental DNA.

摘要

为了在单个 PCR 中检测同时含有 1 型或 2 型光合反应中心的乏氧细菌，我们基于 bchY 基因设计了一套简并引物。新引物通过 GenBank 核苷酸数据库的计算机模拟验证以及对纯菌株和环境 DNA 的 PCR 验证。