基于 BchY 的简并引物可在单个 PCR 中靶向所有类型的厌氧光合细菌。
BchY-based degenerate primers target all types of anoxygenic photosynthetic bacteria in a single PCR.
机构信息
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA.
出版信息
Appl Environ Microbiol. 2009 Dec;75(23):7556-9. doi: 10.1128/AEM.01014-09. Epub 2009 Oct 2.
Abstract
To detect anoxygenic bacteria containing either type 1 or type 2 photosynthetic reaction centers in a single PCR, we designed a degenerate primer set based on the bchY gene. The new primers were validated in silico using the GenBank nucleotide database as well as by PCR on pure strains and environmental DNA.
摘要
为了在单个 PCR 中检测同时含有 1 型或 2 型光合反应中心的乏氧细菌，我们基于 bchY 基因设计了一套简并引物。新引物通过 GenBank 核苷酸数据库的计算机模拟验证以及对纯菌株和环境 DNA 的 PCR 验证。
相似文献
1
BchY-based degenerate primers target all types of anoxygenic photosynthetic bacteria in a single PCR.基于 BchY 的简并引物可在单个 PCR 中靶向所有类型的厌氧光合细菌。
Appl Environ Microbiol. 2009 Dec;75(23):7556-9. doi: 10.1128/AEM.01014-09. Epub 2009 Oct 2.
2
A newly designed degenerate PCR primer based on pmoA gene for detection of nitrite-dependent anaerobic methane-oxidizing bacteria from different ecological niches.一种新设计的基于 pmoA 基因的简并 PCR 引物，用于检测不同生态位的亚硝酸盐依赖型厌氧甲烷氧化菌。
Appl Microbiol Biotechnol. 2013 Dec;97(23):10155-62. doi: 10.1007/s00253-013-5260-8. Epub 2013 Oct 17.
3
Photosynthetic and phylogenetic primers for detection of anoxygenic phototrophs in natural environments.用于检测自然环境中无氧光合生物的光合及系统发育引物。
Appl Environ Microbiol. 2001 Jul;67(7):2922-6. doi: 10.1128/AEM.67.7.2922-2926.2001.
4
Cloning of prokaryotic genes by a universal degenerate primer PCR.利用通用简并引物PCR克隆原核基因
FEMS Microbiol Lett. 2008 Oct;287(2):192-8. doi: 10.1111/j.1574-6968.2008.01311.x. Epub 2008 Aug 21.
5
Putative novel photosynthetic reaction centre organizations in marine aerobic anoxygenic photosynthetic bacteria: insights from metagenomics and environmental genomics.海洋需氧不产氧光合细菌中假定的新型光合反应中心组织：来自宏基因组学和环境基因组学的见解
Environ Microbiol. 2005 Dec;7(12):2027-33. doi: 10.1111/j.1462-2920.2005.00843.x.
6
Metabolic primers for detection of (Per)chlorate-reducing bacteria in the environment and phylogenetic analysis of cld gene sequences.用于检测环境中（过）氯酸盐还原菌的代谢引物及cld基因序列的系统发育分析。
Appl Environ Microbiol. 2004 Sep;70(9):5651-8. doi: 10.1128/AEM.70.9.5651-5658.2004.
7
A New Primer to Amplify pmoA Gene From NC10 Bacteria in the Sediments of Dongchang Lake and Dongping Lake.一种用于扩增东昌湖和东平湖沉积物中NC10细菌pmoA基因的新型引物。
Curr Microbiol. 2017 Aug;74(8):908-914. doi: 10.1007/s00284-017-1260-8. Epub 2017 May 13.
8
Novel polymerase chain reaction primers for the specific detection of bacterial copper P-type ATPases gene sequences in environmental isolates and metagenomic DNA.用于在环境分离物和宏基因组 DNA 中特异性检测细菌铜 P 型 ATP 酶基因序列的新型聚合酶链反应引物。
Lett Appl Microbiol. 2010 Jun 1;50(6):552-62. doi: 10.1111/j.1472-765X.2010.02832.x. Epub 2010 Mar 17.
9
Class P dye-decolorizing peroxidase gene: Degenerated primers design and phylogenetic analysis.P类染料脱色过氧化物酶基因：简并引物设计与系统发育分析
J Microbiol Methods. 2016 Nov;130:148-153. doi: 10.1016/j.mimet.2016.09.016. Epub 2016 Sep 26.
10
Novel acsF Gene Primers Revealed a Diverse Phototrophic Bacterial Population, Including Gemmatimonadetes, in Lake Taihu (China).新型acsF基因引物揭示了太湖（中国）中包括芽单胞菌门在内的多样化光合细菌种群。
Appl Environ Microbiol. 2016 Aug 30;82(18):5587-94. doi: 10.1128/AEM.01063-16. Print 2016 Sep 15.
引用本文的文献
1
Uncovering diversity and abundance patterns of CO-fixing microorganisms in peatlands.揭示泥炭地中固碳微生物的多样性和丰度模式。
NPJ Biodivers. 2025 Aug 4;4(1):30. doi: 10.1038/s44185-025-00099-1.
2
Phylum Gemmatimonadota and Its Role in the Environment.芽单胞菌门及其在环境中的作用。
Microorganisms. 2022 Jan 12;10(1):151. doi: 10.3390/microorganisms10010151.
3
Anoxygenic Phototrophs Span Geochemical Gradients and Diverse Morphologies in Terrestrial Geothermal Springs.无氧光合生物跨越陆地温泉中的地球化学梯度并呈现多种形态。
mSystems. 2019 Nov 5;4(6):e00498-19. doi: 10.1128/mSystems.00498-19.
4
Genomics of Aerobic Photoheterotrophs in Wheat Phyllosphere Reveals Divergent Evolutionary Patterns of Photosynthetic Genes in Methylobacterium spp.小麦叶际好氧光合异养菌的基因组学研究揭示了甲基杆菌属中光合基因的不同进化模式
Genome Biol Evol. 2019 Oct 1;11(10):2895-2908. doi: 10.1093/gbe/evz204.
5
Aerobic Anoxygenic Photosynthesis Is Commonly Present within the Genus Limnohabitans.好氧不产氧光合作用普遍存在于栖湖菌属中。
Appl Environ Microbiol. 2017 Dec 15;84(1). doi: 10.1128/AEM.02116-17. Print 2018 Jan 1.
6
New Dimensions in Microbial Ecology-Functional Genes in Studies to Unravel the Biodiversity and Role of Functional Microbial Groups in the Environment.微生物生态学的新维度——用于揭示环境中功能性微生物群落生物多样性及作用的研究中的功能基因
Microorganisms. 2016 May 24;4(2):19. doi: 10.3390/microorganisms4020019.
7
Novel acsF Gene Primers Revealed a Diverse Phototrophic Bacterial Population, Including Gemmatimonadetes, in Lake Taihu (China).新型acsF基因引物揭示了太湖（中国）中包括芽单胞菌门在内的多样化光合细菌种群。
Appl Environ Microbiol. 2016 Aug 30;82(18):5587-94. doi: 10.1128/AEM.01063-16. Print 2016 Sep 15.
8
Horizontal transfers of two types of puf operons among phototrophic members of the Roseobacter clade.玫瑰杆菌纲光合营养成员中两种类型的puf操纵子的水平转移。
Folia Microbiol (Praha). 2015 Jan;60(1):37-43. doi: 10.1007/s12223-014-0337-z. Epub 2014 Aug 6.
9
Isolation of optically targeted single bacteria by application of fluidic force microscopy to aerobic anoxygenic phototrophs from the phyllosphere.应用流体力显微镜从叶际有氧缺氧光养生物中分离光靶向单细菌。
Appl Environ Microbiol. 2013 Aug;79(16):4895-905. doi: 10.1128/AEM.01087-13. Epub 2013 Jun 14.
10
Abundance and genetic diversity of aerobic anoxygenic phototrophic bacteria of coastal regions of the pacific ocean.太平洋沿海地区好氧型、厌氧型、光合细菌的丰度和遗传多样性。
Appl Environ Microbiol. 2012 Apr;78(8):2858-66. doi: 10.1128/AEM.06268-11. Epub 2012 Feb 3.
本文引用的文献
1
Biogeography and phylogeny of the NOR5/OM60 clade of Gammaproteobacteria.γ-变形菌纲NOR5/OM60进化枝的生物地理学与系统发育
Syst Appl Microbiol. 2009 Apr;32(2):124-39. doi: 10.1016/j.syapm.2008.12.001.
2
The genome of Heliobacterium modesticaldum, a phototrophic representative of the Firmicutes containing the simplest photosynthetic apparatus.适度嗜盐栖热放线菌的基因组，它是厚壁菌门中具有最简单光合装置的光合代表菌。
J Bacteriol. 2008 Jul;190(13):4687-96. doi: 10.1128/JB.00299-08. Epub 2008 Apr 25.
3
Candidatus Chloracidobacterium thermophilum: an aerobic phototrophic Acidobacterium.嗜热嗜酸栖热菌：一种好氧光合嗜酸菌。
Science. 2007 Jul 27;317(5837):523-6. doi: 10.1126/science.1143236.
4
Chlorophyll biosynthesis in bacteria: the origins of structural and functional diversity.细菌中的叶绿素生物合成：结构与功能多样性的起源
Annu Rev Microbiol. 2007;61:113-29. doi: 10.1146/annurev.micro.61.080706.093242.
5
Assessing diversity and biogeography of aerobic anoxygenic phototrophic bacteria in surface waters of the Atlantic and Pacific Oceans using the Global Ocean Sampling expedition metagenomes.利用全球海洋采样探险宏基因组评估大西洋和太平洋表层水中需氧不产氧光合细菌的多样性和生物地理学。
Environ Microbiol. 2007 Jun;9(6):1464-75. doi: 10.1111/j.1462-2920.2007.01265.x.
6
7
Hoeflea phototrophica sp. nov., a novel marine aerobic alphaproteobacterium that forms bacteriochlorophyll a.光养霍夫勒氏菌新种，一种能形成细菌叶绿素a的新型海洋需氧α-变形菌。
Int J Syst Evol Microbiol. 2006 Apr;56(Pt 4):821-826. doi: 10.1099/ijs.0.63958-0.
8
Novel primers reveal wider diversity among marine aerobic anoxygenic phototrophs.新型引物揭示了海洋需氧不产氧光合细菌之间更广泛的多样性。
Appl Environ Microbiol. 2005 Dec;71(12):8958-62. doi: 10.1128/AEM.71.12.8958-8962.2005.
9
Dinoroseobacter shibae gen. nov., sp. nov., a new aerobic phototrophic bacterium isolated from dinoflagellates.石滨玫瑰杆菌属新种，一种从甲藻中分离出的新型需氧光合细菌。
Int J Syst Evol Microbiol. 2005 May;55(Pt 3):1089-1096. doi: 10.1099/ijs.0.63511-0.
10
Prevalence of the Chloroflexi-related SAR202 bacterioplankton cluster throughout the mesopelagic zone and deep ocean.与绿弯菌门相关的SAR202浮游细菌群落在中层带和深海中的分布情况。
Appl Environ Microbiol. 2004 May;70(5):2836-42. doi: 10.1128/AEM.70.5.2836-2842.2004.
Zotero 插件