Heim H K, Oestmann A, Sewing K F
Abteilung Allgemeine Pharmakologie, Medizinische Hochschule Hannover, FRG.
Pharmacology. 1990;40(5):265-70. doi: 10.1159/000138671.
The production of glycoprotein and protein by isolated pig gastric non-parietal cells was measured by incorporation of N-acetyl-[14C]-D-glucosamine [( 14C]GlcNAc) and [3H]-L-leucine [( 3H]Leu), respectively, into acid insoluble material (AIM). Histamine enhanced incorporation of the tracers into cellular and released AIM in a concentration-dependent manner. The H2 receptor antagonist ranitidine completely blocked the effects of histamine (100 mumol/l) on [3H]Leu incorporation into cellular and released AIM (IC50 37 and 32 mumol/l, respectively) but had no inhibitory effect on the 16,16-dimethylprostaglandin-E2 - and forskolin-stimulated tracer incorporation. The H1 receptor antagonist mepyramine did not inhibit the histamine effect. We conclude that histamine is a stimulant of protein, via H2 receptors, and glycoprotein production of isolated pig gastric non-parietal cells.
通过分别将N-乙酰-[14C]-D-葡萄糖胺[（14C）GlcNAc]和[3H]-L-亮氨酸[（3H）Leu]掺入酸不溶性物质（AIM）中，来测定分离的猪胃非壁细胞中糖蛋白和蛋白质的产生。组胺以浓度依赖的方式增强了示踪剂掺入细胞并释放AIM。H2受体拮抗剂雷尼替丁完全阻断了组胺（100μmol/L）对[3H]Leu掺入细胞和释放AIM的作用（IC50分别为37和32μmol/L），但对16,16-二甲基前列腺素-E2和福斯可林刺激的示踪剂掺入没有抑制作用。H1受体拮抗剂美吡拉敏不抑制组胺的作用。我们得出结论，组胺通过H2受体刺激分离的猪胃非壁细胞产生蛋白质和糖蛋白。
