Heim H K, Oestmann A, Sewing K F
Department of Pharmacology, Medical School, Hanover, FRG.
Aliment Pharmacol Ther. 1990 Oct;4(5):477-84. doi: 10.1111/j.1365-2036.1990.tb00494.x.
We studied the effects of prostaglandin (PG) E2, carbenoxolone, cholecystokinin-octapeptide (CCK-OP), and the thromboxane-mimetic U46619, all known to stimulate gastric mucus secretion in vivo, on protein and glycoprotein synthesis in and release from isolated and enriched pig gastric mucous cells, as measured by the incorporation of [3H]L-leucine and N-acetyl-[14C]D-glucosamine respectively into cellular and released acid insoluble material. PGE2 stimulated glycoprotein and protein synthesis (EC50 7 and 30 nmol/L, respectively) and release (EC50 50 and 140 nmol/L, respectively) in a concentration-dependent manner, whereas carbenoxolone, CCK-OP and U46619 failed to enhance the incorporation of the tracers. We conclude that stimulation of mucus secretion by PGE2 is related to direct effects on protein and glycoprotein production of gastric non-parietal cells, whereas indirect effects may be involved in the stimulation by carbenoxolone, CCK-OP, and U46619.
我们研究了前列腺素（PG）E2、生胃酮、八肽胆囊收缩素（CCK-OP）以及血栓素类似物U46619对分离并富集的猪胃黏液细胞中蛋白质和糖蛋白合成及释放的影响，这些物质在体内均已知可刺激胃黏液分泌，分别通过[3H]L-亮氨酸和N-乙酰-[14C]D-葡萄糖胺掺入细胞内及释放的酸不溶性物质来测定。PGE2以浓度依赖方式刺激糖蛋白和蛋白质合成（EC50分别为7和30 nmol/L）及释放（EC50分别为50和140 nmol/L），而生胃酮、CCK-OP和U46619未能增强示踪剂的掺入。我们得出结论，PGE2对黏液分泌的刺激与对胃非壁细胞蛋白质和糖蛋白产生的直接作用有关，而生胃酮、CCK-OP和U46619的刺激可能涉及间接作用。
