Department of Biochemistry, Radboud University Medical Centre, Nijmegen Centre for Molecular Life Sciences, Nijmegen, the Netherlands.
Transfusion. 2010 Feb;50(2):366-75. doi: 10.1111/j.1537-2995.2009.02400.x. Epub 2009 Oct 5.
To maintain a high quality of red blood cells (RBCs), RBC characteristics must be followed during storage under blood bank conditions. By means of infrared (IR) spectroscopy, several characteristics can be measured simultaneously.
IR spectra were acquired for samples from RBCs that were collected and stored according to Dutch blood bank procedures for a period of up to 50 days. Spectra of the soluble cell components were acquired separately after hypotonic lysis of the cells, followed by centrifugation. Characteristic vibrational bands were analyzed with respect to storage time-dependent changes in peak position and in intensity.
A decrease in corresponding peak intensities indicates that RBCs lose protein and lipid during storage. Changes in protein secondary structure during storage are largely confined to integral membrane proteins and membrane-associated proteins. A concurrent decrease in lipid packing density probably reflects the gradual change in cellular shape from discoidal to globular. By integration over a narrow range, storage-dependent changes in intracellular adenosine triphosphate (ATP) and glucose levels could be estimated. ATP levels decrease during storage, but stay above the required 75% of the initial level after 35 days of storage. Glucose concentrations stay well above 5 mmol/L over the entire storage period.
IR spectroscopy is a promising technique to follow structural and metabolic changes in RBCs during storage under blood bank conditions. Several variables can be determined rapidly in a single measurement.
为了保持红细胞（RBC）的高质量，必须在血库条件下储存时跟踪 RBC 的特性。通过红外（IR）光谱，可以同时测量多个特性。
根据荷兰血库程序采集和储存的 RBC 样本，在长达 50 天的时间内获得 IR 光谱。通过对细胞进行低渗裂解并离心后，分别获得可溶细胞成分的光谱。对特征振动带进行分析，以研究其随储存时间的峰位和强度变化。
相应的峰强度下降表明 RBC 在储存过程中失去蛋白质和脂质。储存过程中蛋白质二级结构的变化主要局限于完整的膜蛋白和膜相关蛋白。脂质堆积密度的同时下降可能反映了细胞形状从盘状到球状的逐渐变化。通过在较窄范围内积分，可以估计储存过程中细胞内三磷酸腺苷（ATP）和葡萄糖水平的变化。在储存过程中，ATP 水平下降，但在储存 35 天后仍保持在初始水平的 75%以上。葡萄糖浓度在整个储存期间都保持在 5mmol/L 以上。
IR 光谱是一种很有前途的技术，可以在血库条件下跟踪 RBC 储存过程中的结构和代谢变化。在单次测量中可以快速确定多个变量。
