Zhang Jin-xiang, Xue Tian-yu, Li Hai-xia, Sun Hong-yu, Cheng Jian-ding
Department of Forensic Medicine, Sun Yat-sen College of Medical Science, Zhongshan University, Guangzhou, Guangdong, 510089, PR China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2009 Oct;26(5):580-4. doi: 10.3760/cma.j.issn.1003-9406.2009.05.023.
To investigate the genetic polymorphisms and their forensic application of 9 non-combined of DNA index system (CODIS) short tandem repeat(STR) loci in Guangdong Han population.
DNA samples from 500 unrelated individuals were extracted and amplified with fluorescence labeled multiplex PCR system. PCR products were separated and genotyped with capillary electrophoresis.
One hundred and fifteen alleles and 160 genotypes were observed in the 9 STR loci, respectively. The heterozygosity was 0.824-0.884, the discrimination power (DP) was 0.925-0.969 and the polymorphism information content (PIC) was 0.77-0.86, respectively. The distribution met the Hardy-Weinberg equilibrium (P > 0.05). The total discrimination power was 1.00 x 10(-13), the combined probability of exclusion for trio-paternity testing was 0.999989488. The combined probability of exclusion for duo-paternity testing was 0.873436.
The 9 STR loci are powerful and reliable for personal identification and paternity testing. They can be used as supplementary loci in fatherless (motherless) testing or cases with mutation events.
