Shao Wei-bo, Zhang Su-hua, Li Li
Department of Forensic Medicine, Medical College of Soochow University, Suzhou 215123, China.
Fa Yi Xue Za Zhi. 2011 Feb;27(1):36-8.
To investigate genetic polymorphisms of 21 non-CODIS STR loci in Han population from the east of China and to explore their forensic application value.
Twenty-one non-CODIS STR loci, were amplified with AGCU 21+1 STR kit and DNA samples were obtained from 225 unrelated individuals of the Han population from the east of China. The PCR products were analyzed with 3130 Genetic Analyzer and genotyped with GeneMapper ID v3.2 software. The genetic data were statistically analyzed with PowerStats v12.xls and Cervus 2.0 software.
The distributions of 21 non-CODIS STR loci satisfied the Hardy-Weinberg equilibration. The heterozygosity (H) distributions were 0.596-0.804, the discrimination power (DP) were 0.764-0.948, the probability of exclusion of duo-testing (PEduo) were 0.176-0.492, the probability of exclusion of trios-testing (PEtrio) were 0.334-0.663, and the polymorphic information content (PIC) were 0.522-0.807. The cumulative probability of exclusion (CPE) of duo-testing was 0.999707, the CPE of trios-testing was 0.9999994, and the cumulated discrimination power (CDP) was 0.99999999999999999994.
Twenty-one non-CODIS STR loci are highly polymorphic. They can be effectively used in personal identification and paternity testing in trios cases. They can also be used as supplement in the difficult cases of diad paternity testing.
研究中国东部汉族人群21个非CODIS短串联重复序列（STR）基因座的遗传多态性，并探讨其法医学应用价值。
采用AGCU 21+1 STR试剂盒对21个非CODIS STR基因座进行扩增，从中国东部汉族人群225名无关个体中获取DNA样本。PCR产物用3130基因分析仪进行分析，并用GeneMapper ID v3.2软件进行基因分型。遗传数据用PowerStats v12.xls和Cervus 2.0软件进行统计学分析。
21个非CODIS STR基因座的分布符合Hardy-Weinberg平衡。杂合度（H）分布为0.596 - 0.804，鉴别力（DP）为0.764 - 0.948，单亲检验排除概率（PEduo）为0.176 - 0.492，三联体检验排除概率（PEtrio）为0.334 - 0.663，多态信息含量（PIC）为0.522 - 0.807。单亲检验的累积排除概率（CPE）为0.999707，三联体检验的CPE为0.9999994，累积鉴别力（CDP）为0.99999999999999999994。
21个非CODIS STR基因座具有高度多态性。它们可有效地用于个体识别和三联体亲子鉴定。在二联体亲子鉴定疑难案件中也可作为补充。
