Li Tao, Zhang Yaqing, Zhang Ying'ai
Hainan Huahai Forensic Institute of Judicial Expertise, Haikou, Hainan 570124, China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2021 May 10;38(5):503-505. doi: 10.3760/cma.j.cn511374-20201020-00735.
To investigate the genetic polymorphisms of 21 non-combined DNA index system short tandem repeat (STR) loci in Hainan Li population.
DNA samples from 339 unrelated healthy individuals of Li population from Hainan Province were extracted and amplified with fluorescence labeled multiplex PCR system. PCR products were electrophoresed on an ABI3130 Genetic Analyzer following the manufacturer's instructions. Allele designation was performed with a GeneMapper ID-X by comparison with the allele ladder provided by the corresponding kit.
A total of 173 alleles and 489 genotypes were observed for the 21 STR loci, respectively. The frequencies of alleles and genotypes were 0.0010-0.5434 and 0.0020-0.3274, respectively. The heterozygosity varied from 0.639 to 0.833. Discrimination power (DP) was 0.803-0.948, power of exclusion for trio-paternity was 0.416-0.584, power of exclusion for duo-paternity was 0.140-0.238, the polymorphism information content(PIC) was 0.57-0.81, respectively. The total discrimination power (TDP), cumulative probability of exclusion for trio-paternity testing(CPE-trio) and cumulative probability of exclusion for duo-paternity testing (CPE-duo) were 0.999 999 999 999 99, 0.999 999 883 211 752, and 0.987 266, respectively.
The 21 STR loci are highly polymorphic and informative in the studied population and can be employed as supplementary loci in duo-paternity testing or cases with variant circumstances.
研究海南黎族人群21个非联合DNA索引系统短串联重复序列（STR）基因座的遗传多态性。
提取海南省339名黎族无血缘关系健康个体的DNA样本，采用荧光标记复合PCR系统进行扩增。按照制造商的说明，将PCR产物在ABI3130遗传分析仪上进行电泳。通过与相应试剂盒提供的等位基因阶梯进行比较，使用GeneMapper ID-X进行等位基因指定。
21个STR基因座分别观察到173个等位基因和489种基因型。等位基因频率和基因型频率分别为0.0010 - 0.5434和0.0020 - 0.3274。杂合度在0.639至0.833之间。鉴别力（DP）为0.803 - 0.948，三联体亲权排除率为0.416 - 0.584，二联体亲权排除率为0.140 - 0.238，多态性信息含量（PIC）分别为0.57 - 0.81。总鉴别力（TDP）、三联体亲权检测累积排除概率（CPE-trio）和二联体亲权检测累积排除概率（CPE-duo）分别为0.999 999 999 999 99、0.999 999 883 211 752和0.987 266。
21个STR基因座在研究人群中具有高度多态性和信息性，可作为二联体亲权检测或情况特殊案件的补充基因座。
