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本文引用的文献

1
Chapter 8 Two-dimensional native electrophoresis for fluorescent and functional assays of mitochondrial complexes.第8章 用于线粒体复合物荧光和功能测定的二维天然电泳
Methods Enzymol. 2009;456:153-68. doi: 10.1016/S0076-6879(08)04408-X.
2
Resolving mitochondrial protein complexes using nongradient blue native polyacrylamide gel electrophoresis.使用非梯度蓝色天然聚丙烯酰胺凝胶电泳解析线粒体蛋白质复合物
Anal Biochem. 2009 Jun 15;389(2):143-9. doi: 10.1016/j.ab.2009.03.043. Epub 2009 Apr 5.
3
Respiratory active mitochondrial supercomplexes.呼吸活性线粒体超复合物
Mol Cell. 2008 Nov 21;32(4):529-39. doi: 10.1016/j.molcel.2008.10.021.
4
Electrophoresis techniques to investigate defects in oxidative phosphorylation.用于研究氧化磷酸化缺陷的电泳技术。
Methods. 2008 Dec;46(4):281-7. doi: 10.1016/j.ymeth.2008.09.023. Epub 2008 Oct 21.
5
Features and applications of blue-native and clear-native electrophoresis.蓝色非变性和清亮非变性电泳的特点与应用
Proteomics. 2008 Oct;8(19):3974-90. doi: 10.1002/pmic.200800017.
6
Blue Native electrophoresis to study mitochondrial and other protein complexes.采用蓝色天然电泳研究线粒体及其他蛋白质复合物。
Methods. 2002 Apr;26(4):327-34. doi: 10.1016/S1046-2023(02)00038-5.
7
Blue native polyacrylamide gel electrophoresis: a powerful tool in diagnosis of oxidative phosphorylation defects.蓝色天然聚丙烯酰胺凝胶电泳：诊断氧化磷酸化缺陷的有力工具。
Pediatr Res. 2001 Nov;50(5):658-65. doi: 10.1203/00006450-200111000-00020.
8
Supercomplexes in the respiratory chains of yeast and mammalian mitochondria.酵母和哺乳动物线粒体呼吸链中的超级复合物。
EMBO J. 2000 Apr 17;19(8):1777-83. doi: 10.1093/emboj/19.8.1777.
9
Quantification of muscle mitochondrial oxidative phosphorylation enzymes via histochemical staining of blue native polyacrylamide gels.通过蓝色天然聚丙烯酰胺凝胶的组织化学染色对肌肉线粒体氧化磷酸化酶进行定量分析。
Electrophoresis. 1997 Oct;18(11):2059-64. doi: 10.1002/elps.1150181131.
10
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.

使用蓝色非变性凝胶电泳评估线粒体呼吸链和氧化磷酸化系统。

Evaluation of the mitochondrial respiratory chain and oxidative phosphorylation system using blue native gel electrophoresis.

作者信息

Díaz Francisca, Barrientos Antoni, Fontanesi Flavia

机构信息

University of Miami Miller School of Medicine, Miami, Florida, USA.

出版信息

Curr Protoc Hum Genet. 2009 Oct;Chapter 19:Unit19.4. doi: 10.1002/0471142905.hg1904s63.

DOI:10.1002/0471142905.hg1904s63
PMID:19806591
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2771370/
Abstract

The oxidative phosphorylation (OXPHOS) system consists of five multimeric complexes embedded in the mitochondrial inner membrane. They work in concert to drive the aerobic synthesis of ATP. Mitochondrial and nuclear DNA mutations affecting the accumulation and function of these enzymes are the most common cause of mitochondrial diseases and have also been associated with neurodegeneration and aging. For this reason, several approaches for the assessment of the OXPHOS system enzymes have been progressively developed. Based on methods described elsewhere, the use of blue native gel electrophoresis (BNGE) techniques to routinely assess the OXPHOS system and screen for enzymatic defects in homogenates or mitochondrial preparations from tissues or cultured cells is described here.

摘要

氧化磷酸化（OXPHOS）系统由嵌入线粒体内膜的五种多聚体复合物组成。它们协同工作以驱动ATP的有氧合成。影响这些酶的积累和功能的线粒体和核DNA突变是线粒体疾病的最常见原因，并且也与神经退行性变和衰老有关。因此，已经逐步开发了几种评估OXPHOS系统酶的方法。基于其他地方描述的方法，本文描述了使用蓝色非变性凝胶电泳（BNGE）技术常规评估OXPHOS系统并筛选来自组织或培养细胞的匀浆或线粒体制剂中的酶缺陷。