MRC Mitochondrial Biology Unit, University of Cambridge, Cambridge, UK.
Methods Mol Biol. 2021;2192:287-311. doi: 10.1007/978-1-0716-0834-0_20.
Blue-native polyacrylamide gel electrophoresis (BN-PAGE) is a technique optimized for the analysis of the five components of the mitochondrial oxidative phosphorylation (OXPHOS) system. BN-PAGE is based on the preservation of the interactions between the individual subunits within the integral complexes. To achieve this, the complexes are extracted from the mitochondrial inner membrane using mild detergents and separated by electrophoresis in the absence of denaturing agents. The electrophoretic procedures can then be combined with a variety of downstream detection techniques. Since its development in the 1990s, BN-PAGE has been applied in the study of mitochondria from all kinds of organisms and extensive amounts of data have been produced using this technique, being key for the understanding of many aspects of OXPHOS physiopathology.
蓝色 native 聚丙烯酰胺凝胶电泳 (BN-PAGE) 是一种针对分析线粒体氧化磷酸化 (OXPHOS) 系统的五个组件而优化的技术。BN-PAGE 基于对完整复合物内各个亚基之间相互作用的保留。为此，使用温和的去污剂从线粒体内膜中提取复合物，并在没有变性剂的情况下通过电泳进行分离。然后，可以将电泳程序与各种下游检测技术相结合。自 20 世纪 90 年代发展以来，BN-PAGE 已应用于各种生物体的线粒体研究，并使用该技术产生了大量数据，这对于理解 OXPHOS 病理生理学的许多方面至关重要。
